WormBase Tree Display for RNAi: WBRNAi00099433
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WBRNAi00099433 | Homol | Homol_homol | Y71G12B:RNAi | ||
---|---|---|---|---|---|
Sequence_info | PCR_product | sjj_Y71G12A_197.a | |||
Experiment | Laboratory | DZ | |||
Date | 11 Mar 2010 00:00:00 | ||||
Genotype | rrf-3(pk1426); him-8(e1489); ezIs3 [fkh-6::GFP] | ||||
Treatment | Eggs were recovered from gravid hermaphrodites by hypochlorite treatment, dropped onto RNAi plates, and incubated at 22 degrees Celsius for 3 days. Young adult animals were scored under fluorescence microscopy (Nikon SMZ1500) for missing, abnormal, or sex-inappropriate marker expression. All positive RNAi clones confirmed after retesting were sequenced. | ||||
Delivered_by | Bacterial_feeding | ||||
Inhibits | Predicted_gene | Y71G12B.11a | Inferred_automatically | RNAi_primary | |
Y71G12B.11b | Inferred_automatically | RNAi_primary | |||
Gene | WBGene00006771 | Inferred_automatically | RNAi_primary | ||
Transcript | Y71G12B.11a.1 | Inferred_automatically | RNAi_primary | ||
Y71G12B.11b.1 | Inferred_automatically | RNAi_primary | |||
Species | Caenorhabditis elegans | ||||
Reference | WBPaper00036019 | ||||
Phenotype_not_observed | WBPhenotype:0000961 | Remark | RNAi did not affect expression of the fkh-6::GFP transgene in male animals | ||
Remark | (Table S1) Y71G12B.11 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. | ||||
Method | RNAi |