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WormBase Tree Display for RNAi: WBRNAi00099410

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Name Class

WBRNAi00099410HomolHomol_homolK04D7:RNAi
Sequence_infoPCR_productsjj_K04D7.1
ExperimentLaboratoryDZ
Date11 Mar 2010 00:00:00
Genotyperrf-3(pk1426); him-8(e1489); ezIs3 [fkh-6::GFP]
TreatmentEggs were recovered from gravid hermaphrodites by hypochlorite treatment, dropped onto RNAi plates, and incubated at 22 degrees Celsius for 3 days. Young adult animals were scored under fluorescence microscopy (Nikon SMZ1500) for missing, abnormal, or sex-inappropriate marker expression. All positive RNAi clones confirmed after retesting were sequenced.
Delivered_byBacterial_feeding
InhibitsPredicted_geneK04D7.1Inferred_automaticallyRNAi_primary
GeneWBGene00010556Inferred_automaticallyRNAi_primary
TranscriptK04D7.1.1Inferred_automaticallyRNAi_primary
K04D7.1.3Inferred_automaticallyRNAi_primary
K04D7.1.2Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00036019
Phenotype_not_observedWBPhenotype:0000961RemarkRNAi did not affect expression of the fkh-6::GFP transgene in male animals
Remark(Table S1) rack-1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi