WormBase Tree Display for RNAi: WBRNAi00094607
expand all nodes | collapse all nodes | view schema
WBRNAi00094607 | Homol | Homol_homol | CD4:RNAi | ||
---|---|---|---|---|---|
Sequence_info | PCR_product | sjj_CD4.6 | |||
Experiment | Laboratory | BJS | |||
Date | 12 Jul 2013 00:00:00 | ||||
Genotype | sur-5::UbV-GFP (strain PP563) | ||||
Treatment | Worms treated with 90 Gy IR. | ||||
Delivered_by | Bacterial_feeding | ||||
Inhibits | Predicted_gene | CD4.6 | Inferred_automatically | RNAi_primary | |
Gene | WBGene00003927 | Inferred_automatically | RNAi_primary | ||
Transcript | CD4.6.1 | Inferred_automatically | RNAi_primary | ||
Species | Caenorhabditis elegans | ||||
Reference | WBPaper00044098 | ||||
Phenotype | WBPhenotype:0002010 | Remark | There was no change in sur-5::UbV-GFP reporter expression in RNAi worms after treatment with IR. In wildtype worms the expression is reduced, therefore RNAi resulted in failure to degrade UbV-GFP (protein turnover). sur-5::UbV-GFP reporter expression was assessed 24 h post treatment with 90 Gy IR. | ||
Remark | The sur-5::UbV-GFP reporter strain expresses ubiquitin with an C-terminal glycine to valine mutation fused to GFP under the control of the sur-5 promoter. It is used to assess protein turnover. | ||||
Method | RNAi |