WormBase Tree Display for RNAi: WBRNAi00092769
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WBRNAi00092769 | Homol | Homol_homol | W10C8:RNAi | ||
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Sequence_info | DNA_text | ATGATGGCCGACGAAGAGCTCGGCGATGAGGTGAAGGTGTTCCGTCGGGATGAGGATGCTGACGATGATCCAATGATTAGTGGTGAAACGTCAGAACAACAGTTAGCCGATGATAAAAAAGAAGCTGTAATGGAAGCAGAATTAGACGGTGCCGGTCGAAATCCATCGATTGATGTGTTAAAAAGTGCATTTCCAAAAGTCGAACCAATGTCACCATCGTTTCCCGGTTTAATGTCACACTTCAGTCCTGGATACTCGGCAGCTGCTTTACCCATGTTTATGCCTCTATTTATGAATCCATACGCAGCAGCACTACGATCACCAAGCCTGATGTTTCCAATGGGAGCAATGAGCCCCACATTTCCAATGTTCCCGCCAAGTCCTGTCTATGGAGCAGCAATCGCTGCGGCAGCCGCCAAACAACACTTTGAGAATATGGCTCCACTGAACATGCGAGCCGGTCATCCAATGAATCAGATGGGAATGCCACCATACATGCATCCATCATCAATGGCTCCACAGAATGTCGATCGAAGGGCTCAAGGAGGTGGAAAAGCGAAGAAGGATGATCATGTGAAGAAGCCATTAAACGCGTTCATGTGGTTTATGAAGGAAAATCGAAAAGCACTGCTGGAAGAGATTGGAAATAATGAGAAACAGAGTGCAGAGTTGAATAAAGAGCTTGGAAAGAGGTGGCATGATTTGTCGAAGGAAGAACAGGCGAAATATTTTGAAATGGCAAAGAAGGATAAGGAAACACACAAGGAACGGTATCCGGAGTGGTCGGCGCGGGAAAATTATGCGGTTAATAAGAAAAAGACGAAGAAACGAAGGGATAAGAGTATTCCATCGGAGAACAACGATCAGAAGAAGTGCCGAGCCAGATTCGGAGTTAACAACACAGAAATGTGGTGTAAATTCTGCAAGCGGAAGAAGAAGTGCGAGTACGCAACTGATCGTTCGGGCGGTTCCGATATAACTGACAGTCAGGATGGACGAGGTACAAGTGGTGCGTATAGCAGTAGCTCGGAGAGCCCATCACCAAAGGCAAACGCTGGAATTGCACTGACCACACAGCAGCAGCAAGCAGCAATGATGCATACGATGTTGATGCAAATGCGTCTAGGATCGACGACGGGAGCATCGACGCACGTTCCATCACCACTGGCGTCCTCGTCGGCAGGCAGGAGTCCGCTGGATGCGAACGCGTCGGATTCGGAATCTGATGTTGAGGAGGAGGAAGACGAGCAGATTGATCCGACGGTTATGCAGCAGACACATGATATGCTTATGCAGGAATCGATGTGTACTATTTAA | |||
Experiment | Laboratory | JJ | |||
Date | 01 JAN 1998 00:00:00 | ||||
Genotype | glp-1(e2141); H20-GFP [neuronal marker] | ||||
Delivered_by | Injection | ||||
Inhibits | Predicted_gene | W10C8.2 | Inferred_automatically | RNAi_primary | |
Gene | WBGene00004077 | Inferred_automatically | RNAi_primary | ||
Transcript | W10C8.2.1 | Inferred_automatically | RNAi_primary | ||
Species | Caenorhabditis elegans | ||||
Reference | WBPaper00002998 | ||||
Phenotype | WBPhenotype:0000097 | Remark | glp-1(e2141);pop-1(RNAi) embryos had a much larger amount of hypodermal tissue than did glp-1(e2141) mutant embryos, but few if any neurons, as indicated by the lack of H20-GFP expression. The development of the first two AB daughters was analyzed individually in glp-1(e2141);pop-1(RNAi) embryos by killing all other blastomeres with a laser microbeam. Each AB daughter produced hypodermal cells almost exclusively. The lack of neurons in these experimental embryos indicates that pop-1(+) activity is required for the ABxxa fate. | ||
WBPhenotype:0001312 | Remark | glp-1(e2141);pop-1(RNAi) embryos had a much larger amount of hypodermal tissue than did glp-1(e2141) mutant embryos, but few if any neurons, as indicated by the lack of H20-GFP expression. The development of the first two AB daughters was analyzed individually in glp-1(e2141);pop-1(RNAi) embryos by killing all other blastomeres with a laser microbeam. Each AB daughter produced hypodermal cells almost exclusively. The lack of neurons in these experimental embryos indicates that pop-1(+) activity is required for the ABxxa fate. | |||
WBPhenotype:0001643 | Remark | glp-1(e2141);pop-1(RNAi) embryos had a much larger amount of hypodermal tissue than did glp-1(e2141) mutant embryos, but few if any neurons, as indicated by the lack of H20-GFP expression. The development of the first two AB daughters was analyzed individually in glp-1(e2141);pop-1(RNAi) embryos by killing all other blastomeres with a laser microbeam. Each AB daughter produced hypodermal cells almost exclusively. The lack of neurons in these experimental embryos indicates that pop-1(+) activity is required for the ABxxa fate. | |||
WBPhenotype:0008001 | Remark | glp-1(e2141);pop-1(RNAi) embryos had a much larger amount of hypodermal tissue than did glp-1(e2141) mutant embryos, but few if any neurons, as indicated by the lack of H20-GFP expression. The development of the first two AB daughters was analyzed individually in glp-1(e2141);pop-1(RNAi) embryos by killing all other blastomeres with a laser microbeam. Each AB daughter produced hypodermal cells almost exclusively. The lack of neurons in these experimental embryos indicates that pop-1(+) activity is required for the ABxxa fate. | |||
Remark | (Figure 4, Table 2) pop-1 RNAi. Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation. | ||||
Method | RNAi |