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WormBase Tree Display for RNAi: WBRNAi00092415

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Name Class

WBRNAi00092415HomolHomol_homolY71A12B:RNAi
Y63D3A:RNAi
Sequence_infoDNA_textATGGTGCATTCAAACGGAGCCATCACGAGCACAATTCTGAAGGCTCGTCACGCCGACGTCGTCCGCAAGACGTCACTTCACCACGCCAACGACTTGACACTCATCGATCTCGTGCTCAATGTGCAGCGGCTTTTGGCTCTCCCATCCGACGCGAATTTCGTGTTGAAGTACAAGGATGAAGgtaggaattgcactgaaaattgcgattttaattcaatttttgtcaattttagAAGGCGATTTGGTGACACTCGCCGAGGATAGCGACCTCCTGCTGGCTCTTCACACGTCTGGAGCCACGCTCGACGTGACCGTCGTTGTCGACTCGAGAGCTCGTGAGGCTGTGCACGATGTGCAGAAGCAAGTCGAGCAGATTAAG
ExperimentLaboratoryMSN
Date07 Feb 2011 00:00:00
TreatmentEarly-L4-stage hermaphrodites were soaked in dsRNA for 24 hours at 20 degrees Celsius. Animals were allowed to recover for 48 hours before analysis.
Temperature20
Delivered_bySoaking
InhibitsPredicted_geneY63D3A.5Inferred_automaticallyRNAi_primary
GeneWBGene00006565Inferred_automaticallyRNAi_primary
TranscriptY63D3A.5.2Inferred_automaticallyRNAi_primary
Y63D3A.5.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
InteractionWBInteraction000520199
WBInteraction000520200
ReferenceWBPaper00038310
PhenotypeWBPhenotype:0001403Remarktfg-1 RNAi disrupted normal subcellular localization of both the CAV-1 and SQV-8 proteins in proximal oocytes. These proteins accumulated in the endoplasmic reticulum rather than their normal respective subcellular localizations.
Remark(Figure S2c, S2d) tfg-1 RNAi. Authors report testing three different dsRNA sequences to knockdown tfg-1 and all produced similar results. One selected for curation.
MethodRNAi