WormBase Tree Display for RNAi: WBRNAi00088831
expand all nodes | collapse all nodes | view schema
| WBRNAi00088831 | Homol | Homol_homol | K04D7:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_K04D7.1 | |||
| Experiment | Laboratory | MAD | |||
| Date | 14 Mar 2011 00:00:00 | ||||
| Genotype | [DYN-1::GFP] | ||||
| Treatment | L4-stage hermaphrodites were fed bacteria expressing double-stranded RNA. rack-1(RNAi) experiments were performed for 40-48 hours at 20 degrees C, or 30-36 hours at 25 degrees C. | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | K04D7.1 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00010556 | Inferred_automatically | RNAi_primary | ||
| Transcript | K04D7.1.1 | Inferred_automatically | RNAi_primary | ||
| K04D7.1.3 | Inferred_automatically | RNAi_primary | |||
| K04D7.1.2 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00038375 | ||||
| Phenotype | WBPhenotype:0000679 | Remark | In rack-1(RNAi) embryos, the initial anterior enrichment of DYN-1-GFP foci during establishment phase was not affected (Fig. 3E; also see Video S6). However, during maintenance phase bright DYN-1-GFP puncta expanded into the posterior cortex (Fig. 3F, 7/12 embryos), and sometimes shrunk to a smaller size than that of wild type (2/12 embryos). The expansion/shrinkage was corrected with furrow initiation, and the cleavage furrow was properly placed (Fig. 3G). DYN-1 localization in the two daughter cells was normal, with the exception that in embryos with failed cytokinesis DYN-1-GFP expanded to the posterior cortex after furrow regression (Fig. 3H). We also noticed that in rack-1(RNAi) embryos, the DYN-1-GFP foci were clumping into larger foci than in wild type. | ||
| Remark | rack-1 RNAi | ||||
| Method | RNAi |
