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WormBase Tree Display for RNAi: WBRNAi00066590

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Name Class

WBRNAi00066590HomolHomol_homolY39A1A:RNAi
Sequence_infoPCR_productsjj_Y39A1A.1
ExperimentLaboratoryAQ
Date13 Jun 2005 00:00:00
Genotyperrf-3(pk1426)
TreatmentThree L4 animals were placed onto the RNAi plate, and transferred to a freshly induced plate after 24 h. Nicotine sensitivity of the progeny was scored 96 h later. For candidates causing nicotine sensitivity phenotypes, the RNAi experiment was repeated to verify the finding.
Delivered_byBacterial_feeding
InhibitsPredicted_geneY39A1A.1cInferred_automaticallyRNAi_primary
Y39A1A.1bInferred_automaticallyRNAi_primary
Y39A1A.1aInferred_automaticallyRNAi_primary
Y39A1A.1dInferred_automaticallyRNAi_primary
GeneWBGene00012641Inferred_automaticallyRNAi_primary
TranscriptY39A1A.1d.1Inferred_automaticallyRNAi_primary
Y39A1A.1a.1Inferred_automaticallyRNAi_primary
Y39A1A.1c.1Inferred_automaticallyRNAi_primary
Y39A1A.1b.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00026635
Phenotype_not_observedWBPhenotype:0001573RemarkNicotine sensitivity assays: A total of 30 young adult animals were transferred to NGM plates containing 0.425 or 0.5% (v/v) (-)-nicotine, or 1 or 0.2mM levamisole. Paralysis was followed by visual inspection every 15 minutes and defined as lack of movement in response to prodding.
MethodRNAi