WormBase Tree Display for RNAi: WBRNAi00066586
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| WBRNAi00066586 | Homol | Homol_homol | T20B3:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_T20B3.2 | |||
| Experiment | Laboratory | AQ | |||
| Date | 13 Jun 2005 00:00:00 | ||||
| Genotype | rrf-3(pk1426) | ||||
| Treatment | Three L4 animals were placed onto the RNAi plate, and transferred to a freshly induced plate after 24 h. Nicotine sensitivity of the progeny was scored 96 h later. For candidates causing nicotine sensitivity phenotypes, the RNAi experiment was repeated to verify the finding. | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | T20B3.2 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00006585 | Inferred_automatically | RNAi_primary | ||
| Transcript | T20B3.2.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00026635 | ||||
| Phenotype_not_observed | WBPhenotype:0001573 | Remark | Nicotine sensitivity assays: A total of 30 young adult animals were transferred to NGM plates containing 0.425 or 0.5% (v/v) (-)-nicotine, or 1 or 0.2mM levamisole. Paralysis was followed by visual inspection every 15 minutes and defined as lack of movement in response to prodding. | ||
| Method | RNAi |
