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WBPicture0000013130DescriptionAnalysis of gei-8 expression using transgenic lines. The expression of gei-8 was studied using transgenic lines carrying three different predicted promoters (#1, #2 and #3) fused with gene coding for GFP gei-8::GFP. Panels F, H, I, J and K show the expression from promoter #3. Expression from promoter #2 construct was identical with that from promoter #3 and is not shown. (E and F) Expression of GEI-8::GFP in pharyngeal neurons (arrowheads), ventral nerve cord (arrows), anal sphincter (arrow - as) and tail neurons (arrow - tn) of an L4 larva. (G and H) Expression of GEI-8::GFP in L4 male larva. Additional expression is seen in male specific neurons (arrowheads). (I) L4 larva expressing GEI-8::GFP in egg laying structures, vulval and uterine muscles (arrows), egg laying neurons (arrowheads). (J) GEI-8::GFP expression in somatic muscles (arrows) and nerve cord (arrowheads). (K) Detail of expression of GEI-8::GFP in hermaphrodite tail neuron (arrowhead) and anal sphincter (arrows - as). (Figs. E, G in Nomarski optics and F, H, I, J, K in fluorescence microscopy). Scale: I, J 20 um; E, F, G, H 100 um; K 50 um.
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ReferenceWBPaper00042128