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WBPicture0000011618DescriptionFigure 4. DPY-26, but not DPY-27, is present in the germline and is associated with meiotic chromosomes. (A) The posterior half of a wild-type XX L4 worm stained with propidium iodide (red) and anti-DPY-26 (green) to create the merged image shown. The left half of the image shows the gonad. DPY-26 colocalizes with all chromosomes in the germ cell nuclei (yellow arrow) but is localized only to the X chromosomes (white arrows) in somatic cells. Maturing sperm (green arrow) indicate the proximal end of the gonad. Scale bar, 20 um. (B to D) Germ cell nuclei in pachytene were stained with anti-DPY-26 (green) (B) and propidium iodide (red) (C). A merged image is shown in (D). (E and F) A wild-type XX embryo (E) and a wild-type XX larva (F) were stained with anti-DPY-27 (green) and anti-P-granule (red) specific to germline precursor cells and germ cells (24) to show that DPY-27 is absent from the germline. Scale bar, 10 um.
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ReferenceWBPaper00002605