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WBPicture0000011120 Description: Figure 2. A vem-1 reporter gene is expressed in a small subset of neurons that extend longitudinally projecting axons into the right VNC in C. elegans embryos and larvae. A-C, vem-1::GFP is expressed in embryonic neurons before the comma stage (A), at the 1.5-fold stage (B), and at the threefold stage (C). Red arrowheads point to vem-1::GFP-expressing head neurons. Expression of vem-1::GFP in the AVG and ventral nerve cord (nc) is noted in B and in the ventral nerve cord in C. D, vem-1::GFP expression is associated with a subset of nerve ring neurons, as well as the AVG at the L1 stage (lateral view). Neurons were identified based on their relative positions. Inset, Bottom left, A corresponding Nomarski optics image indicating the position of the nerve ring. E, In L4 worms, vem-1::GFP expression is detected in the AVG and within a small subset of nerve ring-associated neurons that extend axons into the right VNC. The brightly labeled nerve ring is located at the right, or anterior, end of the worm. The expression of vem-1::GFP in AVG is consistent with the presence of GFP in the dorsalmost axon (see AVG axon indicated by long blue arrows) of the VNC (short blue arrows), which characteristically extends beyond its VNC-associated neighbors to the extreme posterior end of the animal. The additional axonal labeling (short blue arrows) reflects the expression of vem-1::GFP in nerve ring-associated neurons that compose more ventral regions of the VNC. The animal depicted in this micrograph was selected because it provided a particularly good example of GFP labeling along the extent of AVG- and VNC-associated axons. However, this was one of the small subset of animals in which we also observed non-neuronal labeling. F, In L4 worms, a translational reporter, consisting of the full-length VEM-1 protein fused to GFP driven by the vem-1 promoter, revealed VEM-1::GFP expression in a subset of nerve ring neurons that extend axons into the VNC (blue arrow), as well as the AVG (blue arrow). Note that the non-neuronal labeling that was variably observed in the transcriptional reporter line was mostlyabsent in the VEM-1::GFP translational line. Scale bars, 10 um.
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  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2004
    Article_URL: DOI id 10.1523/JNEUROSCI.2385-04.2004
    Journal_URL: TheJournalofNeuroscience
    Publisher_URL: SocietyofNeuroscience
  Reference: WBPaper00024484