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WBPicture0000010165 | Description | Fig. 6. Localization of a SMG-4:GFP fusion. (A) Graphic depiction of the GFP fusion construct generated in pgm185 at a unique site of the final intron. (B) 10 overview and 40 zoom of a fully paralyzed smg-4(r1181);unc-54(r293)::SMG4-GFP (qaEx5) animal, visualized by both brightfield and fluorescence microscopy. (C) 63 images of a live smg-4 (r1181);unc-54 (r293)::SMG4-GFP (qaEx5) embryo visualized fluorescence microscopy. The line from C points to a bright punctum of GFP fluorescence in the cytoplasm of one cell, while the line from N points to a bright punctum in the same cell overlying accumulated nuclear signal. Beneath is a graphic depiction of the marked part of this image, with rough outlines of two spindle shaped myocytes in white and two nuclei in light gray. |
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Name | F6.jpg | |
Depict (3) | ||
Acknowledgment (5) | ||
Reference | WBPaper00004705 |