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WBPicture0000008093DescriptionFigure 6. Expression of the CePAK mRNA during development. A, blot containing total RNA from six C. elegans developmental stages (E, L1 to L4, and A) was subjected to Northern blot analysis. The autoradiogram was exposed at 70 C for 4 days. B, the same filter was stripped and probed with a 32P-labeled actin cDNA (23). The autoradiogram was exposed at 70 C overnight. C, signals from autoradiograms were quantified separately by scanning densitometry in an Ultroscan XL laser densitometer (Pharmacia Biotech Inc.). The data are normalized to the integrated intensities of signals of C. elegans actin mRNA obtained at each developmental stage. The relative abundance (%) of both mRNAs at each stage is shown.
NameF6.large.jpg
DepictExpr_patternExpr1597
Acknowledgment (5)
ReferenceWBPaper00002544