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| WBPicture0000008085 | Description | Figure 3. Expression pattern analysis of ceh-6 using anti-CEH-6 antibodies (A-E,H-L). Affinity-purified anti-CEH-6 antibodies were incubated with whole-mount fixed mixed-stage C. elegans and visualized using anti-rabbit FITC-coupled secondary antibodies. Expression was also analyzed using reporter constructs: transgenic animals with the lacZ reporter construct LZ6-1 were visualized by staining with X-gal (F ,G) using differential interference contrast (DIC), the GFP reporter construct GFP6-1 was visualized using fluorescent microscopy (M,N). (A) Ventral view of the head of an L1 animal: expression of CEH-6 in the ring ganglia of the head. Specifically marked are the excretory cell (ex) and the SABV neurons. The dots' to the right of the SABV neurons are background from the pharyngeal lumen. (B) DAPI of A. (C) Dorsal focal plane; CEH-6 expression in AVH. (D) Same animal as in C; subventral focal plane; CEH-6 expression in RMDV and AUA. (E) Lateral view of CEH-6 expression. (F,G) ceh-6::lacZ expression in head region, two different focal planes are shown. Excretory canals are stained despite a nuclear localization signal; twisted appearance is due to the rol-6 marker used to generate transgenic animals. The dorsal focal plane shows the neurons RMDDL/R and AVHL/R, the excretory canals are also visible as twisting lines. The ventral focal plane shows RMDVL/R, AVHL/R, the excretory cell and canals, and SABVL/R. (H) CEH-6 expression in the rectal cells. (I) DAPI of same animal as in H. (J) CEH-6 expression in the ventral nerve cord. Transient expression is seen in the dividing Pn.a cells. Note that P11.a and P12.a are just undergoing cell division, CEH-6 is localized in the cytoplasm in these cells. (K) DAPI of same animals as in J. (L) CEH-6 expression in the ventral nerve cord at a stage slight prior to that in J; the posterior Pn.a cells have not yet divided. (M,N) ceh-6::gfp expression in the comma stage embryo, DIC (M), and fluorescent channel (N). Note that most cells that express ceh-6 in the head are localized where close to each other at that stage (arrows point to the clusters in the head region and the tail region). (O) Schematic summary overview of the ceh-6 expressing cells in a late L1 larva. | |
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| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 2001 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00004617 |
