WormBase Tree Display for Interaction: WBInteraction000556222
expand all nodes | collapse all nodes | view schema
WBInteraction000556222 | Interaction_type | Physical | ProteinProtein | ||
---|---|---|---|---|---|
Interactor | Interactor_overlapping_gene | WBGene00001194 | Interactor_type | Bait | |
WBGene00003218 | Interactor_type | Target | |||
Interaction_summary | "EGL-27(1-1129) was used to screen 3.4 x 10^6 cDNAs, and eight positive cDNAs were identified; three of these cDNAs corresponded to MEP-1. A fragment of MEP-1-containing residues 364-870, which includes all seven zinc-finger domains, was sufficient to interact with full-length EGL-27 in yeast. In addition, MEP-1 residues 330-870 were sufficient to interact with the amino-terminal 637 residues of EGL-27 in yeast (data not shown)."; "The interaction of MEP-1 with full-length EGL-27 was demonstrated to be a direct interaction by a GST-pull-down assay (Figure 5D)" | ||||
Detection_method | Affinity_capture_Western | ||||
Throughput | Low_throughput | ||||
WBProcess | WBbiopr:00000020 | ||||
Paper | WBPaper00046298 |