WormBase Tree Display for Interaction: WBInteraction000549517
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| WBInteraction000549517 | Interaction_type | Regulatory | Change_of_expression_level | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00008205 | Interactor_type | Trans_regulator | |
| WBGene00000190 | Interactor_type | Trans_regulated | |||
| Interaction_summary | "We validated expression profiling results by quantitative RT-PCR of selected genes normalized to act-1 levels after sams-1(RNAi) (not shown), as well as in sams-1(ok3033) animals (Figures S1B and S1C). We also normalized qPCR validations to an external "spiked-in" RNA (JNK1a1 alpha, gift of Dr. Roger Davis). These results were similar to the microarray and to the act-1 normalized qPCR for upregulated (Figures 1C and 1D), downregulated (Figure 1E), or unchanged genes (Figure 1F) tested." | ||||
| Detection_method | RT_PCR | ||||
| Regulation_level | Transcriptional | ||||
| Paper | WBPaper00048490 | ||||
| Remark | Fig. 1c |
