WormBase Tree Display for Interaction: WBInteraction000502388
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| WBInteraction000502388 | Interaction_type | Regulatory | Change_of_expression_level | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00004932 | Interactor_type | Trans_regulated | |
| Transgene | WBTransgene00001052 | ||||
| Construct | WBCnstr00001043 | ||||
| WBGene00004800 | Interactor_type | Trans_regulator | |||
| Interaction_summary | In sir-2.1-overexpressing strains, the GFP signal was much stronger than in the wild-type, indicating that transcription from the sod-3 promoter was upregulated. | ||||
| Detection_method | Construct | ||||
| Reporter_gene | [sod-3::gfp] | ||||
| Transgene | |||||
| Regulation_level | Transcriptional | ||||
| Paper | WBPaper00027682 | ||||
| Remark | A loss-of-function mutation in daf-16 eliminated this upregulation of sod-3, suggesting that it results from the activation of DAF-16 by the elevated levels of SIR-2.1. The upregulation of sod-3 by the sir-2.1-overexpressing transgene was abolished by deletion of ftt-2 as well. Thus, overexpression of SIR-2.1 appears to promote DAF-16 transcriptional activity and requires 14-3-3 to do so. |
