WormBase Tree Display for Interaction: WBInteraction000501562
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| WBInteraction000501562 | Interaction_type | Regulatory | |||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00004374 | Interactor_type | Trans_regulated | |
| Expr_pattern | Expr3456 | ||||
| Antibody | WBAntibody00000259 | ||||
| WBAntibody00000260 | |||||
| WBGene00000776 | Interactor_type | Trans_regulator | |||
| Variation_interactor | WBVar00091657 | Interactor_type | Trans_regulator | ||
| Interaction_summary | In immunofluorescence studies, an antibody to RME-2 localises in a punctate pattern at the surface of late stage oocytes. In one- and two-cell stage embryos, RME-2 can be detected in intracellular vesicles; it rapidly disappears as embryos develop, presumably as it is no longer required and is degraded following its release from the yolk ligand. cpl-1(ok360) oocytes and early embryos showed a similar punctate pattern of anti-RME-2 reactivity to that in wild-type embryos. However, instead of rapidly disappearing, RME-2 could still be detected in later stage mutant embryos, in which it localised to the periphery of the large yolk vesicles. Localisation of RME-2 to the peripheral region and yolk to the lumen, indicated that although present in the same compartment, yolk and RME-2 are separated from one another. RME-2 localisation could be observed in terminal stage mutant embryos, although the level of receptor was slightly decreased. This suggested that recycling and/or degradation of the yolk receptor is significantly delayed, but not completely blocked, in cpl-1 mutant embryos. | ||||
| Detection_method | Antibody | ||||
| Regulation_result | Negative_regulate | Life_stage | WBls:0000005 | ||
| Paper | WBPaper00024412 |
