| WBGene00001949 | SMap | S_parent | Sequence | CHROMOSOME_I |
|---|
| Identity | Version | 1 |
| | Name | CGC_name | hlh-2 | Person_evidence | WBPerson346 |
| | | Sequence_name | M05B5.5 |
| | | Molecular_name | M05B5.5a |
| | | | M05B5.5a.1 |
| | | | CE06191 |
| | | | M05B5.5b |
| | | Other_name | CELE_M05B5.5 | Accession_evidence | NDB | BX284601 |
| | | Public_name | hlh-2 |
| | DB_info | Database (12) |
| | Species | Caenorhabditis elegans |
| | History | Version_change | 1 | 07 Apr 2004 11:29:26 | WBPerson1971 | Event | Imported | Initial conversion from geneace |
| | Status | Live |
| Gene_info | Biotype | SO:0001217 |
| | Gene_class | hlh |
| | Allele (33) |
| | Possibly_affected_by | WBVar02154068 |
| | Legacy_information | [C.elegansII] NMK. Encodes homolog of vertebrate E12, Drosophila daughterless. lacZ fusion indicates transcription in some neuronal precursors. Antibody staining indicates all blastomere nuclei stain up to 200-cell stage, later expression complex, strong in head, ventral cord and tail at comma stage; fewer than 20 cells stain at hatching. Cosmid K07G5. [KM] |
| | | [Portman DS] bx108 and bx115 enhance the male tail ray-loss phenotype of lin-32 alleles, but have no noticeable phenotype in a wild-type background |
| | Strain (6) |
| | RNASeq_FPKM (74) |
| | GO_annotation (76) |
| | Ortholog (43) |
| | Structured_description | Concise_description | hlh-2 encodes a Class I basic helix-loop-helix (bHLH) transcription factor that is the C. elegans ortholog of the mammalian E and Drosophila Daughterless transcriptional activators; HLH-2 activity is required for cell fate specifications occuring during embryonic and larval development that affect such processes as gonadogenesis, male tail formation, and programmed cell death; HLH-2 has been shown to dimerize with at least two C. elegans Acheate-scute homologs, LIN-32, a neural-specific protein with which it functions in male tail development and HLH-3, with which it is coexpressed in the nuclei of embryonic neuronal prescursors and with which it regulates the transcription of the EGL-1 cell death activator in the NSM sister cells; in gonadogenesis, HLH-2 is required for bestowing proAC competence on the cells that undergo the AC/VU (anchor cell/ventral uterine precursor) cell fate decision, for specification, differentiation, and function of the distal tip cell (DTC) and AC, including transcriptional regulation of the LAG-2 Delta-like ligand in the latter, and for formation of the uterine seam cell (utse); genetic analysis also suggests that HLH-2 functions with HLH-14, an additional Acaete-scute homolog, to specify the PVQ/HSN/PHB neuroblast cell lineage; HLH-2 is expressed in all nuclei of early embryos until the ~200-cell stage, when expression becomes increasingly restricted to neuronal cells and their immediate precursors; later expression is detected in, but not limited to, pharyngeal cells, anterior neurons, vulval and uterine muscles, the DTCs, the presumptive and mature AC, the Q neuroblast, and enteric muscles; comparative analysis of transcriptional and translational reporters indicates that hlh-2 is expressed in both the anchor cell and the ventral uterine (VU) precursor, but that expression in the latter is subject to post-transcriptional down-regulation; HLH-2 accumulation in the presumptive AC is the first detectable difference between the AC and VU precursors during the lateral specification event that distinguishes these two cell fates. | Paper_evidence | WBPaper00002826 |
| | | | | | WBPaper00004492 |
| | | | | | WBPaper00006120 |
| | | | | | WBPaper00006303 |
| | | | | | WBPaper00006354 |
| | | | | | WBPaper00024330 |
| | | | | Curator_confirmed | WBPerson1843 |
| | | | | Date_last_updated | 26 Aug 2004 00:00:00 |
| | | Automated_description | Enables several functions, including DNA-binding transcription factor activity, RNA polymerase II-specific; bHLH transcription factor binding activity; and protein dimerization activity. Contributes to RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Involved in several processes, including generation of neurons; regulation of metabolic process; and sex differentiation. Located in cytoplasm and nucleus. Part of RNA polymerase II transcription regulator complex. Expressed in several structures, including distal tip cell; enteric muscle; lateral ganglion; ray precursor cell; and terminal bulb. Used to study obesity. Human ortholog(s) of this gene implicated in several diseases, including Fuchs' endothelial dystrophy; Pitt-Hopkins syndrome; and agammaglobulinemia (multiple). Is an ortholog of human TCF12 (transcription factor 12); TCF3 (transcription factor 3); and TCF4 (transcription factor 4). | Paper_evidence | WBPaper00065943 |
| | | | | Curator_confirmed | WBPerson324 |
| | | | | | WBPerson37462 |
| | | | | Inferred_automatically | This description was generated automatically by a script based on data from the WS291 version of WormBase |
| | | | | Date_last_updated | 29 Nov 2023 00:00:00 |
| Disease_info | Experimental_model | DOID:9970 | Homo sapiens | Paper_evidence | WBPaper00061889 |
| | | | | Curator_confirmed | WBPerson324 |
| | | | | Date_last_updated | 28 Jun 2022 00:00:00 |
| | Potential_model | DOID:3883 | Homo sapiens | Inferred_automatically | Inferred by orthology to human genes with DO annotation (HGNC:11634) |
| | | DOID:9256 | Homo sapiens | Inferred_automatically | Inferred by orthology to human genes with DO annotation (HGNC:11633) |
| | | DOID:2340 | Homo sapiens | Inferred_automatically | Inferred by orthology to human genes with DO annotation (HGNC:11623) |
| | | DOID:0090070 | Homo sapiens | Inferred_automatically | Inferred by orthology to human genes with DO annotation (HGNC:11623) |
| | | DOID:0060488 | Homo sapiens | Inferred_automatically | Inferred by orthology to human genes with DO annotation (HGNC:11634) |
| | | DOID:10283 | Homo sapiens | Inferred_automatically | Inferred by orthology to human genes with DO annotation (HGNC:11633) |
| | | DOID:11555 | Homo sapiens | Inferred_automatically | Inferred by orthology to human genes with DO annotation (HGNC:11634) |
| | | DOID:0081140 | Homo sapiens | Inferred_automatically | Inferred by orthology to human genes with DO annotation (HGNC:11633) |
| | | DOID:0081143 | Homo sapiens | Inferred_automatically | Inferred by orthology to human genes with DO annotation (HGNC:11633) |
| Models_disease_in_annotation | WBDOannot00001265 |
| Molecular_info | Corresponding_CDS | M05B5.5a |
| | Corresponding_transcript | M05B5.5b |
| | | M05B5.5a.1 |
| | Corresponding_pseudogene_history | M05B5.5b:wp122 |
| | Other_sequence (12) |
| | Associated_feature (17) |
| | Gene_product_binds (22) |
| | Transcription_factor | WBTranscriptionFactor000080 |
| | | WBTranscriptionFactor000063 |
| | | WBTranscriptionFactor000078 |
| | | WBTranscriptionFactor000076 |
| | | WBTranscriptionFactor000082 |
| | | WBTranscriptionFactor000083 |
| | | WBTranscriptionFactor000079 |
| | | WBTranscriptionFactor000084 |
| | | WBTranscriptionFactor000077 |
| | | WBTranscriptionFactor000081 |
| Experimental_info | RNAi_result (80) |
| | Expr_pattern (20) |
| | Drives_construct (20) |
| | Construct_product (15) |
| | Regulate_expr_cluster | WBPaper00059477:hlh-2(RNAi)_regulated |
| | Antibody | WBAntibody00000147 |
| | | WBAntibody00001214 |
| | | WBAntibody00001643 |
| | Microarray_results (21) |
| | Expression_cluster | WBPaper00028788:MPK-1_Response_Weak |
| | | WBPaper00040285:H2S_24hr_upregulated |
| | | WBPaper00044260:nicotic-acid_downregulated |
| | | WBPaper00044656:tatn-1(qd182)_downregulated |
| | | WBPaper00044736:flat_dev_expression |
| | | WBPaper00044760:germline_specific |
| | | WBPaper00044786:emr-1(RNAi);lem-2(tm1582)_downregulated |
| | | WBPaper00045521:Oogenic |
| | | WBPaper00045618:puf-8_downregulated |
| | | WBPaper00045729:gld-2(RNAi)_downregulated |
| | | WBPaper00046338:body_muscle_unique |
| | | WBPaper00046497:B.thuringiensis_0.1mix_downregulated_12h |
| | | WBPaper00046497:B.thuringiensis_0.5mix_downregulated_6h |
| | | WBPaper00046497:B.thuringiensis_0.5mix_downregulated_12h |
| | | WBPaper00046691:AB_enriched |
| | | WBPaper00046741:Garlic-Extract_upregulated_mRNA |
| | | WBPaper00046805:csr-1(RNAi)_downregulated_Set1 |
| | | WBPaper00048923:Aging_regulated |
| | | WBPaper00048988:neuron_expressed |
| | | WBPaper00049498:npr-1(ur89)_regulated_3 |
| | | WBPaper00049626:UNC-75_target |
| | | WBPaper00049942:HeatShock_upregulated_hsf-1(RNAi) |
| | | WBPaper00049942:hsf-1(RNAi)_upregulated |
| | | WBPaper00050488:adult_vs_dauer_regulated_N2_20C |
| | | WBPaper00050712:RC301_vs_DA650_4h-PA_upregulated |
| | | WBPaper00050726:OsmoticStress_regulated_Food |
| | | WBPaper00050726:starvation_regulated_LowSalt |
| | | WBPaper00050859:upregulated_P-granule(-)GFP(+)_vs_control_day2-adult |
| | | WBPaper00051039:germline_enriched |
| | | WBPaper00051265:F1_hrde-1(tm1200)_downregulated |
| | | WBPaper00051265:F4_hrde-1(tm1200)_upregulated |
| | | WBPaper00051265:F4_morc-1(tm6048)_upregulated |
| | | WBPaper00051558:fog-2(q71)_regulated |
| | | WBPaper00052884:emb-4(hc60)_downregulated |
| | | WBPaper00053184:sma-2(rax5)_downregulated |
| | | WBPaper00053184:sma-4(rax3)_downregulated |
| | | WBPaper00053295:lin-22(icb38)_upregulated |
| | | WBPaper00053295:lin-22(ot269)_upregulated |
| | | WBPaper00053321:lin-15B(RNAi)_downregulated_starved-L1_Truseq |
| | | WBPaper00053321:nos-1(gv5)nos-2(RNAi)_upregulated_fed-L1_Truseq |
| | | WBPaper00053321:PGCs_depleted_NuGen |
| | | WBPaper00053599:female_vs_male_upregulated |
| | | WBPaper00053599:mitosis_vs_meiosis_downregulated |
| | | WBPaper00053599:oocyte_vs_mitosis_downregulated |
| | | WBPaper00055013:hmg-3(bar24)_upregulated |
| | | WBPaper00055386:daf-2(e1370)_downregulated |
| | | WBPaper00055565:AVK_enriched |
| | | WBPaper00055626:pry-1(mu38)_upregulated |
| | | WBPaper00055648:germline_expressed |
| | | WBPaper00055971:nhl-2(ok818)_20C_upregulated |
| | | WBPaper00055971:nhl-2(ok818)_25C_upregulated |
| | | WBPaper00056090:E.faecalis_downregulated_hpx-2(dg047) |
| | | WBPaper00056161:hermaphrodite_enriched_siRNA |
| | | WBPaper00056167:Cry5B_upregulated |
| | | WBPaper00056443:sek-1(km4)_upregulated |
| | | WBPaper00056471:aak-1(tm1944);aak-2(ok524)_upregulated |
| | | WBPaper00056826:hmc_biased |
| | | WBPaper00057288:Cold_downregulated_transcript |
| | | WBPaper00059224:tph-1(mg280)_upregulated |
| | | WBPaper00059471:EGL-43_interacting |
| | | WBPaper00059477:hlh-2(RNAi)_regulated |
| | | WBPaper00059664:srbc-48(ac23)_downregulated |
| | | WBPaper00059728:5ug_cholesterol_downregulated |
| | | WBPaper00059728:20ug_cholesterol_downregulated |
| | | WBPaper00060459:bcat-1(RNAi)_downregulated |
| | | WBPaper00060634:fhod-1(tm2363)_upregulated |
| | | WBPaper00060661:sensory-neuron_depleted |
| | | WBPaper00060683:hlh-11(ko1)_downregulated |
| | | WBPaper00060764:swsn-1(os22ts)_downregulated |
| | | WBPaper00060811:L1_vs_adult_upregulated_neural |
| | | WBPaper00060909:atfs-1(cmh15)_upregulated |
| | | WBPaper00061007:HB101_downregulated |
| | | WBPaper00061007:S.aquatilis_downregulated |
| | | WBPaper00061340:ABarpaaa_lineage |
| | | WBPaper00061340:AIN_parent |
| | | WBPaper00061340:AMso_parent |
| | | WBPaper00061340:DB |
| | | WBPaper00061340:Excretory_cell_parent |
| | | WBPaper00061340:g1A_parent |
| | | WBPaper00061340:g2_parent |
| | | WBPaper00061340:GLR |
| | | WBPaper00061340:hmc |
| | | WBPaper00061340:hmc_and_homolog |
| | | WBPaper00061340:hmc_homolog |
| | | WBPaper00061340:I1_parent |
| | | WBPaper00061340:I2_grandparent |
| | | WBPaper00061340:Intestinal_and_rectal_muscle |
| | | WBPaper00061340:M4_parent |
| | | WBPaper00061340:mc3 |
| | | WBPaper00061340:Neuroblast_I6_M5 |
| | | WBPaper00061340:Neuroblast_M2_M3 |
| | | WBPaper00061340:Parents_of_U_F_B_DVA |
| | | WBPaper00061340:Parents_of_Y_DA6_DA7_DA9 |
| | | WBPaper00061340:Parent_of_AMsh_URB |
| | | WBPaper00061340:Parent_of_g1P_I3 |
| | | WBPaper00061340:Parent_of_hyp3_ILsoDx |
| | | WBPaper00061340:Parent_of_PVP_and_rect_V |
| | | WBPaper00061340:Parent_of_tail_spike_and_hyp10 |
| | | WBPaper00061340:Pharyngeal_intestinal_valve |
| | | WBPaper00061340:Pharyngeal_neuron |
| | | WBPaper00061340:rect_D |
| | | WBPaper00061340:RIC |
| | | WBPaper00061340:RIC_parent |
| | | WBPaper00061340:URA_parent |
| | | WBPaper00061424:Diet_MYb120_downregulated |
| | | WBPaper00061439:unc-30(ok613)_downregulated_P.aeruginosa-infection |
| | | WBPaper00061527:nrde-3-tra-4_10484 |
| | | WBPaper00061527:utx-1_2886-swan-1_2192 |
| | | WBPaper00061613:human-amyloid-beta_alpha-Synuclein(A53T)_upregulated |
| | | WBPaper00061651:ADL_enriched |
| | | WBPaper00061651:ASH_enriched |
| | | WBPaper00062118:neural-HSF-1_upregulated |
| | | WBPaper00062159:hda-2(ok1479)_downregulated |
| | | WBPaper00062325:muscle_depleted_coding-RNA |
| | | WBPaper00062456:doxycycline_downregulated_CB4856_transcript |
| | | WBPaper00062456:doxycycline_downregulated_N2_transcript |
| | | WBPaper00062585:hlh-26(ok1453)_downregulated_E.faecium |
| | | WBPaper00062587:lsl-1(ljm1)_downregulated |
| | | WBPaper00062587:lsl-1(tm4769)_downregulated |
| | | WBPaper00064088:Day-1-adult_vs_L4_downregulated_fem-3(q20) |
| | | WBPaper00064088:Day-1-adult_vs_L4_downregulated_glp-1(e2141) |
| | | WBPaper00064088:Day-3-adult_vs_L4_downregulated_daf-16(mu86);glp-1(e2141) |
| | | WBPaper00064088:Day-3-adult_vs_L4_downregulated_fem-3(q20) |
| | | WBPaper00064088:Day-3-adult_vs_L4_downregulated_glp-1(e2141) |
| | | WBPaper00064229:B.thuringiensis-Cry5Ba_downregulated |
| | | WBPaper00064602:egl-9(sa307)_upregulated |
| | | WBPaper00064716:paraquat_upregulated |
| | | WBPaper00064769:him-17(me24)_downregulated |
| | | WBPaper00065096:25C_vs_20C_upregulated |
| | | WBPaper00065096:Day10_vs_Day1_upregulated |
| | | WBPaper00065158:25C_vs_15C_upregulated_WholeAnimal |
| | | WBPaper00065258:anchor-cell_enriched |
| | | WBPaper00065315:mating_upregulated_Day2-7 |
| | | WBPaper00065835:Day11_vs_Day1_upregulated |
| | | WBPaper00065841:1_0 |
| | | WBPaper00065841:1_2 |
| | | WBPaper00065841:7_4 |
| | | WBPaper00065841:11_0 |
| | | WBPaper00065841:31_0 |
| | | WBPaper00065841:41_1 |
| | | WBPaper00065841:47_1 |
| | | WBPaper00065841:48_0 |
| | | WBPaper00065841:74_0 |
| | | WBPaper00065841:78_0 |
| | | WBPaper00065841:81_0 |
| | | WBPaper00065841:102_0 |
| | | WBPaper00065841:103_0 |
| | | WBPaper00065841:110_0 |
| | | WBPaper00065841:110_1 |
| | | WBPaper00065841:143_0 |
| | | WBPaper00065926:pgph-2(overepxreesion)_upregulated_glucose |
| | | WBPaper00065975:P-body_vs_oocyte_enriched |
| | | WBPaper00065975:P-body_vs_WholeAnimal_enriched |
| | | WBPaper00065993:glp-1(e2141)_downregulated |
| | | WBPaper00066062:xrep-4(lax137)_upregulated |
| | | WBPaper00066110:Doxorubicin_downregulated_diploid |
| | | WBPaper00066110:tetraploid_vs_diploid_downregulated |
| | | WBPaper00066146:germline-inx-14(RNAi)_downregulated_PA14 |
| | | WBPaper00066204:CdCl2_downregulated_mRNA |
| | | cgc4386_cluster_5_2 |
| | | WBPaper00025032:cluster_7 |
| | | WBPaper00025032:cluster_68 |
| | | WBPaper00025141:N2_Expressed_Genes |
| | | WBPaper00025141:unc-4::GFP_Expressed_Genes |
| | | WBPaper00026929:sir-2.1_overexpression_regulated |
| | | WBPaper00031003:0hr_muscle_depleted |
| | | WBPaper00031003:24hr_muscle_depleted |
| | | WBPaper00031003:total_muscle_depleted |
| | | WBPaper00034661:AgNPs_upregulated |
| | | WBPaper00035588:alg-1(-)_upregulated |
| | | WBPaper00035873:dpy-9_regulated |
| | | WBPaper00035873:dpy-10_regulated |
| | | WBPaper00035905:FBF-1_Associated |
| | | WBPaper00036123:Atrazine_regulated |
| | | WBPaper00036383:ash-2_Day8_regulated |
| | | WBPaper00037695:CC_3-day |
| | | WBPaper00040963:Q100_down |
| | | WBPaper00040963:Q200_down |
| | | WBPaper00041606:CE_X.nematophila_regulated |
| | | WBPaper00041939:control_vs_UVC-EtBr-exposed_51h |
| | | WBPaper00041939:UVC-EtBr-exposed_vs_UVC-exposed_51h |
| | | WBPaper00041960:N2_fasting_upregulated_anytime |
| | | WBPaper00042236:flcn-1(ok975)_upregulated |
| | | WBPaper00042258:DA1877_downregulated_GravidAdult |
| | | WBPaper00045263:0.1mM-paraquat_upregulated |
| | | WBPaper00045263:isp-1(qm150)_upregulated |
| | | WBPaper00045263:nuo-6(qm200)_upregulated |
| | | WBPaper00045263:ProLongevity-mtROS_upregulated |
| | | WBPaper00048989:N2_rapamycin_downregulated |
| | | WBPaper00053236:Starvation_regulated_N2 |
| | | WBPaper00056330:mrps-5(RNAi)_downregulated |
| | | WBPaper00057098:SiO2-nanoparticles_upregulated |
| | | [cgc5767]:cluster_43 |
| | | [cgc5767]:expression_class_E |
| | | [cgc5767]:expression_class_E_pi(41_min) |
| | | [cgc5767]:expression_class_E_pi(53_min) |
| | | [cgc5767]:expression_class_M |
| | | [cgc5767]:expression_class_ME |
| | | [cgc6390]:Cluster_E |
| | | [cgc6390]:oogenesis-enriched |
| | Interaction (108) |
| Map_info | Map | I | Position | 1.83482 | Error | 0.002317 |
| | Well_ordered |
| | Positive | Positive_clone | C01H6 |
| | | | F21E8 |
| | | | M05B5 | Inferred_automatically | From sequence, transcript, pseudogene data |
| | Mapping_data | Multi_point | 3837 |
| | | | 3838 |
| | | | 3839 |
| | | | 3840 |
| | | | 3841 |
| | | | 3842 |
| | | | 4521 |
| | | | 4760 |
| Reference (133) |
| Method | Gene |
