WormBase Tree Display for Expr_pattern: Expr976
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| Expr976 | Expression_of | Gene | WBGene00001148 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001148 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000041 | |||
| WBls:0000019 | |||
| WBls:0000021 | |||
| Anatomy_term (21) | |||
| Type | Reporter_gene | GFP expression was analyzed in ncIs1 hermaphrodites carrying p5E5 as a stable integrated transgenic array. Though p5E5 contains a 2-kb genomic fragment derived from LGV at the 5' side in addition to the fragment corresponding to the eat-20 gene, it is confirmed that an extrachromosomal array of p5E5 and that of p5E5del, in which the 2-kb fragment was deleted, gave the same GFP expression pattern. Thus, the fragment at the 5' part of the insert had no effects on the expression of GFP. Neurons expressing GFP were first identified in L1 larvae. | |
| Pattern | In embryos, GFP expression was first detected at the 2-fold stage. Sometimes eight cells in the anterior half of the body expressed GFP. At the 3-fold stage, several cells around the pharynx expressed GFP in most embryos. About half of the embryos also expressed GFP weakly in the pharynx. At L1, GFP was detected in a subset of neurons. About half of the larvae also expressed GFP in the pharynx; expression in the terminal bulb was the most intense. At the adult stage, GFP was detected in the pharyngeal muscles, m3, m4, and m6. In addition, GFP was detected in a subset of neurons: IL1, OLQ, BAG, and ALN, several circumpharyngeal cells, and coelomocytes. Very faint GFP fluorescence was detected in the pharyngeal neurons including I4 and I5 in L1 larvae. | ||
| Picture | WBPicture0000011717 | ||
| Remark | Reporter gene fusion type not specified. | ||
| The antibody staining showed EAT-20 expression in the seam cells and hypodermal cells, which is inconsistent with the absence of GFP expression in those cells in the promoter trap lines, suggesting that p5E5 may not contain all the cis-regulatory elements necessary for the expression of the eat-20 gene. | |||
| Reference | WBPaper00003907 | ||
| Transgene | WBTransgene00001248 |
