WormBase Tree Display for Expr_pattern: Expr9225
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| Expr9225 | Expression_of (2) | |
|---|---|---|
| Expression_data (3) | ||
| Subcellular_localization | mCherry-SAM-10 signal became detectable as early as the 1.5-fold stage during embryogenesis. However, SAM-10 is not nuclearly localized at this stage. Using RAB-3-GFP as a marker for PLMs, it was observed that SAM-10 was not nuclearly localized until the late embryonic threefold stage, just before hatching. Thus, SAM-10 translocated into the nucleus in embryonic animals, well before PLM synaptic branches have even formed in early larval stage animals, indicating that SAM-10 nuclear localization is not driven by pre- and postsynaptic cell contact. | |
| Type | Reporter_gene | |
| Pattern | The mCherry-SAM- 10 signal was detected broadly throughout the animal with dense expression in the head ganglion,vulva and tail ganglion.sam-10 expression was observed in PLM neurons. Furthermore, using GFP-RAB-3 as a nuclear-excluded marker in the PLM soma, was found that SAM- 10 was nuclearly localized in the PLM. The expression of was characterized using an mCherry-sam- 10 fusion transgene that fully rescues the sam-10(js94) phenotypes. | |
| Reference | WBPaper00037849 | |
| Transgene | WBTransgene00030991 |
