WormBase Tree Display for Expr_pattern: Expr9038
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| Expr9038 | Expression_of | Gene | WBGene00007547 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00007547 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000019 | ||||
| WBls:0000020 | ||||
| WBls:0000021 | ||||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0004510 | Certain | |||
| WBbt:0004511 | Certain | |||
| WBbt:0005451 | Certain | |||
| WBbt:0005772 | Certain | |||
| WBbt:0005798 | Certain | |||
| WBbt:0006751 | Certain | |||
| Type | Reporter_gene | |||
| Pattern | The expression of nhr-154 GFP reporter transgenes was first detected in the 2-fold stage embryo within the developing pharynx and in precursors of several unidentified head neurons. By the 3-fold stage of embryogenesis, expression was seen in the pharynx and throughout the intestine, a pattern reminiscent of the developmental transcription factor pha-4. In L1 and L2 stages, the reporter gene expression was strong in the pharyngeal muscles (anterior and posterior bulbs, predominantly), in unidentified head neurons, the intestine, and in the intestinal-rectal valve or sphincter cell. GFP reporter gene expression decreased in subsequent larval stages (L3, L4) so that by the adult stage only pharyngeal expression persistence is reproducibly observed. This suggested that the peak of expression in adults detected by RT-qPCR might be due to the embryos inside gravid adults in these samples. No obvious differences in pattern were seen between the two promoter lengths tested. The expression of nhr-154 GFP reporter was not observed in male specific structures. | |||
| Remark | Picture: Fig 5A, 5B, 5C. | |||
| Reference | WBPaper00036257 | |||
| Transgene | WBTransgene00030870 |
