WormBase Tree Display for Expr_pattern: Expr4681
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| Expr4681 | Expression_of | Gene | WBGene00000382 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000382 | ||
| Expression_data | GO_term | GO:0005737 | |
| GO:0000794 | |||
| Subcellular_localization | In contrast to the partial translocation of endogenous CDC-6, transgenic CDC-6::GFP appeared to localize completely to the cytoplasm during S phase. CDC-6::GFP was present throughout the cell cycle and localized to condensed chromosomes during mitosis. | ||
| Type | Reporter_gene | ||
| Picture | WBPicture0000009842 | ||
| Remark | To determine the time course of CDC-6 nuclear export during S phase, authors investigated CDC-6::GFP or CDC-6::tdTomato localization at set time points. At 120 to 135 min after hatch, CDC-6::GFP remained nuclear in most of the seam cells except V5. Cytoplasmic localization was first detected at 150 to 165 min after hatch for V2 to V4, at 165 to 180 min for V6, and at 195 to 210 min for V1. The timing of CDC-6 translocation for the seam cells correlated with the timing of S phase entry. | ||
| To determine whether the exclusive cytoplasmic localization of CDC-6::GFP during S phase resulted from the degradation of nuclear CDC-6::GFP, authors created a constitutively nuclear-localized CDC-6::GFP by adding two SV40 large T antigen nuclear-localization signals (NLSs) to the C terminus and inactivating the predicted nuclear-export signal (NES) through L434A and L436A amino acid subsitutions. This CDC-6mNES+2NLS protein had stable nuclear-protein levels throughout the cell cycle, indicating that CDC-6 does not undergo appreciable degradation in the nucleus during S phase. | |||
| Reference | WBPaper00029399 | ||
| Transgene | WBTransgene00029380 |
