WormBase Tree Display for Expr_pattern: Expr4283
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| Expr4283 | Expression_of | Gene | WBGene00006543 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006543 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| WBls:0000010 | ||||
| WBls:0000013 | ||||
| Anatomy_term | WBbt:0003737 | Certain | ||
| WBbt:0003739 | Certain | |||
| WBbt:0003740 | Certain | |||
| WBbt:0004302 | Certain | |||
| WBbt:0005439 | Certain | |||
| WBbt:0005813 | Certain | |||
| GO_term | GO:0005634 | |||
| GO:0005737 | ||||
| Subcellular_localization | While TBX-2::GFP was initially localized to nuclei, it was detected in the cytoplasm as well as the nucleus in pm4 and pm5 cells by the 1.5-fold stage. Cytoplasmic expression was not homogeneous. Rather, TBX-2::GFP appeared filamentous, as though it was associated with the cytoskeleton. Cytoplasmic expression was observed even in lines expressing very low levels of TBX-2::GFP, suggesting that cytoplasmic TBX-2::GFP did not reflect over-expression from transgenes. The same pattern was observed in tbx-2(ok529); tbx-2::GFP embryos. This localization pattern suggests that the timing of tbx-2 transcriptional function likely initiates before the 1.5-fold stage, when TBX-2 appears completely nuclear. | |||
| Type | Reporter_gene | |||
| Pattern | TBX-2::GFP expression was present in the both MS- and ABa-derived pharynx cells. TBX-2::GFP expression initiated at the 8E stage (staging by the number of endodermal, or E, cells), in 11 - 12 anteriorly localized pharyngeal cells. Based on position, these cells are likely to be the ABa descendants that will give rise to pharyngeal muscle cells (i.e., ABalpaaa a/p, ABalpapp a/p, ABaraaaaa, ABaraapa a/p, ABarapaa a/p, ARarapapp and ABaraapp a/p). By the 1.5-fold stage, TBX-2::GFP was expressed in pm3, pm5 and variably pm4, with expression persisting throughout the larval and adult stages. Surprisingly, expression extended beyond the ABa lineage after the 1.5-fold stage. For example, only 2/6 pm5 nuclei derive from ABa but authors often observed all pm5 cells expressing TBX-2::GFP in larvae. By the 3-fold stage authors also observed expression in pharyngeal neurons, occasionally in the posterior muscle pm8 and in cells outside of the pharynx such as body wall muscles. Thus, TBX-2::GFP expression initiated within the ABa lineage but ultimately appeared in both ABa and MS-derived muscle cells. | |||
| Reference | WBPaper00028546 | |||
| Transgene | WBTransgene00029095 |
