WormBase Tree Display for Expr_pattern: Expr3808
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| Expr3808 | Expression_of | Gene | WBGene00003990 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003990 | |||
| Expression_data | Life_stage | WBls:0000041 | ||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0005319 | Certain | |||
| WBbt:0005772 | Certain | |||
| Type | Antibody | |||
| Pattern | Not detected in embryos via immunostaining. Expressed in adult worms. Immuno-fluorescent staining of PFN-2 was strong in the intestinal wall, the spermatheca, and the pharynx. | |||
| Remark | Although anti-PFN-2 and anti-PFN-3 antibodies showed specific reactivity for respective isoform on Western blot of total C. elegans lysates, they had some reactivity to null mutants in immunofluorescent staining. Therefore, these antibodies were additionally adsorbed to acetone fixed powders of pfn-2 and pfn-3 null mutant worms respectively to ascertain the specificity of these antibodies. These treatments significantly reduced nonspecific staining of the respective null mutant. | |||
| The patterns of expression and localization of PFN-2 in the pfn-3 null mutants were indistinguishable from those in wild type. | ||||
| Reference | WBPaper00026958 | |||
| Antibody_info | WBAntibody00001033 |
