WormBase Tree Display for Expr_pattern: Expr3807

Expr3807 Expression_of: Gene: WBGene00003991
    Reflects_endogenous_expression_of: WBGene00003991
  Expression_data: Life_stage: WBls:0000024
      WBls:0000038
      WBls:0000027
      WBls:0000035
      WBls:0000041
      WBls:0000018
      WBls:0000019
      WBls:0000020
      WBls:0000021
    Anatomy_term: WBbt:0005813 Certain
    GO_term: GO:0055120
  Subcellular_localization: Immunostaining showed PFN-3 was specifically expressed in body wall muscle and localized in a striking dot-like fashion in the dense bodies. When the dense bodies were viewed vertically to the plasma membrane, PFN-3 colocalized with a-actinin and vinculin. However, when they were observed laterally, PFN-3 partially colocalized with a-actinin and localized adjacent to vinculin. Since vinculin is closer to the plasma membrane than that of a-actinin in the dense bodies, PFN-3 is located at the cytoplasmic tips of the dense bodies. As expected, these dot-like staining patterns of PFN-3 were absent in the pfn-3 null mutants.
  Type: Antibody
  Pattern: Staining for PFN-3 was negative in early embryos but detected specifically in body wall muscle cells from the 1.5-fold stage (~350 min after the first cell division) and persisted through embryogenesis. Also expressed in adult worms.
  Remark: Although anti-PFN-2 and anti-PFN-3 antibodies showed specific reactivity for respective isoform on Western blot of total C. elegans lysates, they had some reactivity to null mutants in immunofluorescent staining. Therefore, these antibodies were additionally adsorbed to acetone fixed powders of pfn-2 and pfn-3 null mutant worms respectively to ascertain the specificity of these antibodies. These treatments significantly reduced nonspecific staining of the respective null mutant.
    The patterns of expression and localization of PFN-3 in the pfn-2 were indistinguishable from those in wild type.
  Reference: WBPaper00026958
  Antibody_info: WBAntibody00001034