WormBase Tree Display for Expr_pattern: Expr3765
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Expr3765 | Expression_of | Gene | WBGene00002987 | |
---|---|---|---|---|
Reflects_endogenous_expression_of | WBGene00002987 | |||
Expression_data | Anatomy_term | WBbt:0003828 | Certain | |
WBbt:0003829 | Certain | |||
WBbt:0006483 | Certain | |||
WBbt:0006638 | Certain | |||
Type | Reporter_gene | |||
Pattern | To examine lim-4 expression during the embryogenesis, the eggs were stained at various developing stages with anti-myc antibodies. A GFP reporter was used for the GATA gene elt-2, which is specifically expressed in the gut lineage, to stage the embryos. MYC::LIM-4 immunoreactivity is first detected in two cells approximately 160 min post-fertilization at 8 gut nuclei stage. The two MYC::LIM-4-staining cells was identified as the neuroblasts ABalpppppa and ABpraaappa that respectively give rise to left/right ADF and AWB neurons, based on triple labeling of the embryos with antibodies raised against the LIN-26 protein, which is expressed in the nuclei of the hypodermal cells that border the ADF/AWB mother cells. Like lim-4::gfp, the MYC::LIM-4 immunoreactivity is not detected in ADF at any stage of larvae or adults, but it is observed in AWB and several other neurons in the head throughout life (N > 200). It is difficult to determine if LIM-4 is excluded from ADF or it is present there transiently. | |||
Remark | This myc::lim-4 transgene potently rescues ADF serotonergic phenotype in yz12 mutants, indicating that the MYC::LIM-4 protein is expressed in cells necessary to specify ADF serotonergic identity. | |||
Reference | WBPaper00026818 | |||
Transgene | WBTransgene00005170 |