WormBase Tree Display for Expr_pattern: Expr3762
expand all nodes | collapse all nodes | view schema
Expr3762 | Expression_of | Gene | WBGene00001400 |
---|---|---|---|
Reflects_endogenous_expression_of | WBGene00001400 | ||
Expression_data | Life_stage | WBls:0000024 | |
WBls:0000003 | |||
WBls:0000038 | |||
WBls:0000027 | |||
WBls:0000035 | |||
WBls:0000041 | |||
WBls:0000015 | |||
WBls:0000021 | |||
Anatomy_term (23) | |||
GO_term | GO:0005634 | ||
Subcellular_localization | Expressed in the nuclei. | ||
Type | Antibody | ||
Pattern | FAX-1 protein was detected in the nuclei of wild-type embryonic neurons beginning at mid-embryogenesis (approximately 350 min), the time at which most embryonic neurons differentiate, and through all larval and adult stages. For most neurons, the expression pattern was static throughout larval and adult stages. The pattern, location, and number of neurons that expressed FAX-1 in the embryo were also consistent with the postembryonic expression. This analysis confirmed the strong expression of FAX-1 in the nuclei of AVK interneurons previously reported using different methods. In addition, FAX-1 protein accumulated to high levels in the nuclei of AVA, AVB, and AVE bilateral interneuron pairs of the head. Strong FAX-1 expression was also identified in the RIC bilaterally-paired interneurons. FAX-1 was strongly expressed in two additional bilateral neuron pairs located just ventral to the AVA/AVE neurons. These neurons are SIBD and either SIBV or SMBV. Strong expression of FAX-1 protein was observed in the M4 motorneuron of the pharynx. Weak and variable FAX-1 expression was observed in the AIY interneuron pair of young larva, but no expression in adults. The only other neuron that accumulated FAX-1 was the unpaired DVA interneuron of the tail. FAX-1 protein was not observed at any stage in any of the 69 ventral midline motorneurons that mediate body movements. FAX-1 protein expressed in a dynamic fashion in the nuclei of two non-neuronal cell types during larval development. FAX-1 protein was detected in the paired distal tip cells (DTCs) of the hermaphrodite somatic gonad of L2 through L4 stage larvae and two pairs of vulval cells in L4 hermaphrodite larvae. During the time of FAX-1 expression, both of these cell types are undergoing either long range migration (DTCs) or complex local morphogenesis (vulval cells). | ||
Remark | In contrast to the findings of fax-1::gfp expression analysis, no accumulation of FAX-1 protein was observed in neurons of the retro-vesicular ganglion of the head or preanal ganglion of the tail, the CEP sensory neurons, or the dorsal rectal cell. Either the gfp analysis yielded these cells as false positives due to transgenic artifacts, or the gfp reporter analysis was more sensitive than anti-FAX-1 antibody. The alternative explanation that the anti-FAX-1 antibody only detects some isoforms of FAX-1 is unlikely because cDNA analysis has revealed no evidence of alternative splicing outside of the unconserved N-terminus and because the antibodies we used were polyclonal and raised to the entire known FAX-1 protein. | ||
Reference | WBPaper00026828 | ||
Antibody_info | WBAntibody00000945 |