WormBase Tree Display for Expr_pattern: Expr3470
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| Expr3470 | Expression_of | Gene | WBGene00006321 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006321 | |||
| Homol | Homol_homol | T22B2:Expr | ||
| Expression_data | Anatomy_term | WBbt:0003681 | Certain | |
| WBbt:0005813 | Certain | |||
| GO_term | GO:0005634 | |||
| Subcellular_localization | GFP-tagged SUP-12, which was expressed in the body wall muscle under the control of the myo-3 promoter, predominantly localized to the nuclei. Within the nuclei, GFP-SUP-12 was found in diffuse and often speckled patterns in the nucleoplasm but excluded from the globular region, possibly representing the nucleolus. The speckled localization of SUP-12 in the nucleus is similar to patterns that are commonly observed for other splicing factors. | |||
| Type | Reporter_gene | |||
| Pattern | The 3.1-kb promoter region of the sup-12 gene was able to drive expression of a reporter GFP in body wall muscle and pharynx. | |||
| Picture | WBPicture0000008970 | |||
| Remark | Although the myo-3 promoter is active in the body wall muscle but not in the pharynx, myo-3 driven expression of GFP-SUP-12 was sufficient to rescue the Sup-12 mutant phenotype, indicating that GFP-SUP-12 is functional and expression of SUP-12 in the body wall muscle is functionally important for its interaction with unc-60. These results strongly suggest that SUP-12 is a muscle-specific regulator of pre-mRNA splicing in the nucleus. | |||
| Reference | WBPaper00024604 | |||
| Transgene | WBTransgene00028624 |
