WormBase Tree Display for Expr_pattern: Expr2553
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| Expr2553 | Expression_of (2) | ||
|---|---|---|---|
| Expression_data (3) | |||
| Subcellular_localization | The DPY-7 protein is detected in circumferential bands within the cuticle of each larval stage and the adult. The DPY-7 bands locate within the furrows that delineate the annuli. The DPY-7 bands are not continuous around the entire worm body. In the adult and the L1 larva, longitudinal ridges termed alae exist within the cuticle above the seam cells. DPY-7 was not detected within these ridges or the matrix region immediately surrounding them. In larval stages with no alae, the DPY-7 bands start and end above the lateral seam cells where they partly interdigitate. When a partial reduction step is included during fixation for immunostaining, the DPY-7containing bands dissociate from other ECM material and adopt a discrete thread-like appearance. The DPY-7 bands seem to be more resistant to reduction than the ECM material that lies between the bands. DPY-7 collagen probably assembled into tight band or thread-like structures that run circumferentially around the body of the animal, located within the furrows that delineate the annuli. | ||
| Type | Antibody | Mouse monoclonal antibody against DPY-7. Recombinant DPY-7 protein fragments were generated in Escherichia coli by using the QIAGEN vector pQE-30, according to standard manufacturer's protocol. A DPY-7 fragment constituting most of the predicted mature protein was used to immunize mice, and monoclonal cell lines were generated by fusing splenocytes to Sp2/O-Ag14 myeloma cells by using standard methods. The cell line DPY7-5a was selected for use based on the sensitivity and specificity of the antibody for the DPY-7 protein. It reacts to an epitope within the 40 carboxy-terminal non-Gly-X-Y residues of the protein. | |
| Pattern (2) | |||
| Picture (2) | |||
| Reference | WBPaper00005815 | ||
| Antibody_info | WBAntibody00000615 |
