WormBase Tree Display for Expr_pattern: Expr2321
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| Expr2321 | Expression_of | Gene | WBGene00003911 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003911 | |||
| Expression_data | Life_stage | WBls:0000003 | ||
| Anatomy_term | WBbt:0005733 | Certain | ||
| GO_term | GO:0005886 | |||
| Subcellular_localization | cell boundaries | |||
| Type | Antibody | Affinity-purified anti-CePAK polyclonal rabbit antibody. | ||
| Pattern | Whereas no signal was detected in early embryogenesis where cell proliferation takes place, CePAK was specifically localized at all hypodermal cell boundaries throughout the second phase of the embryogenesis. The identity of these cells as hypodermal cells was supported by the identical staining pattern of the MH27 monoclonal antibody, which exclusively recognizes hypodermal cell boundaries. During the elongation event, boundaries of the hypodermal cells disappear progressively as the distance between the longitudinal margins of each cell increase, whereas that between the circumferential margins decrease. The CePAK staining pattern coincided with these cell shape changes, as illustrated from an embryo at the beginning of elongation when all hypodermal cell boundaries were still present to an elongated embryo where only two continuous longitudinal boundaries were observed. | |||
| Picture | WBPicture0000008095 | |||
| Remark | As a negative control, no signal was detected in similar immunofluorescence analysis using the CePAK antibody, which was blocked by the GST/CePAK fusion protein. | |||
| Reference | WBPaper00002544 | |||
| Antibody_info | WBAntibody00000121 |
