WormBase Tree Display for Expr_pattern: Expr1632
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| Expr1632 | Expression_of | Gene | WBGene00000453 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000453 | ||||
| Expression_data | Life_stage | WBls:0000041 | |||
| WBls:0000023 | |||||
| WBls:0000015 | |||||
| WBls:0000021 | |||||
| WBls:0000010 | |||||
| WBls:0000013 | |||||
| Anatomy_term | WBbt:0003995 | Certain | |||
| WBbt:0003997 | Certain | ||||
| WBbt:0004679 | Partial | ||||
| Remark | Expressed in the first ventral nuclei of hyp6. | ||||
| WBbt:0004685 | Certain | ||||
| WBbt:0004687 | Certain | ||||
| WBbt:0004690 | Certain | ||||
| WBbt:0005105 | Partial | ||||
| Remark | Expressed in a pair of neuron cells in dorsal and lateral ganglion | ||||
| WBbt:0005298 | Partial | ||||
| Remark | Expressed in a pair of neuron cells in dorsal and lateral ganglion | ||||
| WBbt:0005375 | Partial | ||||
| Remark | Expressed in 12 cells in anterior ganglion. | ||||
| WBbt:0005828 | Certain | ||||
| GO_term | GO:0005634 | ||||
| Subcellular_localization | The CEH-32 protein is detected exclusively in the nuclei. | ||||
| Type | Antibody | A specific rabbit antiserum raised against the carboxy-terminal part of the CEH-32 protein was used in immunostaining experiments to confirm and complete the expression pattern. The specificity of this antibody was demonstrated by lack of staining after preincubation with the immunizing antigen. | |||
| Reporter_gene | |||||
| Pattern | Although the antibody and reporter patterns coincide in a number of key aspects, a few differences are evident. Expression in the head hypodermal cells upon hatching is only faintly detected with the gfp reporter construct. Moreover, only a few neurons in the head express the ceh-32::gfp construct, compared with the immunostaining results. These differences could reflect that positive control elements are missing or inactive in the reporter transgenes. | ||||
| The expression of ceh-32 begins during the embryonic development, during the gastrulation stage (in 100-min-old embryos), and persists until adults. Embryonic expression of ceh-32 is detected in the anterior part of the embryos in head hypodermal and neuronal precursor cells. Upon hatching, ceh-32 is expressed in the hypodermal and neuronal cells of the head as well as in the somatic gonad. The head hypodermal nuclei expressing CEH-32 were identified as the nuclei of hyp3, hyp4, hyp5, and the first ventral nuclei of hyp6. In the head neurons, ceh-32 is found expressed in 12 cells of the anterior ganglion, in the sensory neurons ADL, in a pair of neurons in the dorsal side of lateral and ventral ganglion, and in 8 neurons in the ventral side of the lateral and ventral ganglion. Some weakly expressing cells were not identified. In the somatic gonad, the expression of ceh-32 begins at the L1/L2 stage in the sheath/spermatheca (SS) precursor cells, and this expression is maintained during the development of the somatic gonad in the gonadal sheath cells. In the adult worm, all the gonadal sheath cells express ceh-32. | |||||
| Reference | WBPaper00004825 | ||||
| Transgene | WBTransgene00000295 | ||||
| Antibody_info | WBAntibody00000396 |
