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WormBase Tree Display for Expr_pattern: Expr1415

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Name Class

Expr1415Expression_ofGeneWBGene00003220
Reflects_endogenous_expression_ofWBGene00003220
Expression_dataLife_stageWBls:0000002
Anatomy_termWBbt:0004575Certain
WBbt:0004576Certain
WBbt:0005772Certain
WBbt:0005784Certain
GO_termGO:0005634
GO:0005737
GO:0005694
GO:0005654
Subcellular_localizationnuclei, nucleoplasm, cytoplasmic, associated with the chromosomes
TypeAntibodyAffinity-purified rabbit polyclonal antibodies against the amino-terminal half of MES-2.
PatternMES-2 is highly enriched in the germline and is located in the nuclei. All germ nuclei in the mitotic and meiotic regions are stained, with the exception of sperm. A maternal load of protein is seen in the nucleoplasm of oocytes; mature sperm lack detectable protein. Adult males also display staining in germ nuclei, which declines during spermatogenesis and is undetectable in sperm. The only adult somatic nuclei that contain detectable levels of MES-2 are those in the intestine. Staining in intestinal nuclei is extremely faint in some samples and undetectable in other samples. In early embryos, MES-2 is observed in the nuclei of all blastomeres. In blastomeres that lack an intact nuclear envelope because they are undergoing mitosis, MES-2 is predominantly cytoplasmic, but some MES-2 is clearly associated with the chromosomes. At progressively later stages of embryogenesis, the intensity of MES-2 staining in the nuclei of somatic cells decreases. Upon hatching, staining of MES-2 is significantly higher in the nuclei of the two primordial germ cells, Z2 and Z3, than in somatic nuclei. At later stages of larval development, MES-2 is oberved exclusively in the germline and localized in the nuclei.
PictureWBPicture0000007984
WBPicture0000007985
ReferenceWBPaper00003144
Antibody_infoWBAntibody00000184