WormBase Tree Display for Expr_pattern: Expr13520
expand all nodes | collapse all nodes | view schema
| Expr13520 | Expression_of | Gene | WBGene00004157 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004157 | ||||
| Expression_data | Life_stage | WBls:0000041 | Anatomy_term | WBbt:0003681 | |
| WBbt:0005788 | |||||
| WBls:0000023 | Anatomy_term | WBbt:0003681 | |||
| WBbt:0005788 | |||||
| WBls:0000020 | Anatomy_term | WBbt:0003681 | |||
| WBbt:0005788 | |||||
| WBls:0000021 | Anatomy_term | WBbt:0003681 | |||
| WBbt:0005788 | |||||
| Anatomy_term | WBbt:0003681 | Partial | |||
| Life_stage | WBls:0000020 | ||||
| WBls:0000021 | |||||
| WBls:0000023 | |||||
| WBls:0000041 | |||||
| Remark | Posterior pharynx. | ||||
| WBbt:0005788 | Partial | ||||
| Life_stage | WBls:0000020 | ||||
| WBls:0000021 | |||||
| WBls:0000023 | |||||
| WBls:0000041 | |||||
| Remark | Posterior pharynx. | ||||
| Not_in_Anatomy_term | WBbt:0005784 | ||||
| Type | Genome_editing | ||||
| Reporter_gene | |||||
| Pattern | Extremely faint expression was only observed in the posterior pharynx. To amplify the PQN-75:: GFP::3xFLAG signal, worms were fixed and stained green with M2 anti-flag and a blue DAPI/DNA costain, and still there was no evidence of germline expression. PQN-75 staining was exclusively in the pharynx, starting in the threefold stage of embryogenesis, becoming progressively more pronounced through larval development. Within the pharynx, PQN-75 was most abundant in the pharyngeal gland cells and could be observed in gland-cell processes that extend along the pharyngeal lumen. Interestingly, PQN-75 staining was not detected within the pharyngeal lumen, buccal cavity, or on the anterior cuticle. Instead, 77% (n=200) of larval-staged worms had varying amounts of PQN-75 aggregates throughout the pharynx, suggesting that gland cells secrete PQN-75 into the surrounding pharyngeal muscle. | ||||
| Reference | WBPaper00053071 | ||||
| Variation | WBVar02148626 |
