WormBase Tree Display for Expr_pattern: Expr12265
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| Expr12265 | Expression_of | Gene | WBGene00006649 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006649 | ||||
| Expression_data | Life_stage | WBls:0000041 | Anatomy_term | WBbt:0003732 | |
| WBbt:0005319 | |||||
| WBbt:0005772 | |||||
| WBbt:0006865 | |||||
| WBls:0000023 | Anatomy_term | WBbt:0003732 | |||
| WBbt:0005319 | |||||
| WBbt:0005772 | |||||
| WBbt:0006865 | |||||
| WBls:0000006 | |||||
| WBls:0000007 | |||||
| WBls:0000008 | |||||
| WBls:0000017 | Anatomy_term | WBbt:0006749 | |||
| Anatomy_term | WBbt:0003732 | Certain | |||
| Life_stage | WBls:0000023 | ||||
| WBls:0000041 | |||||
| WBbt:0005319 | Certain | ||||
| Life_stage | WBls:0000023 | ||||
| WBls:0000041 | |||||
| WBbt:0005772 | Certain | ||||
| Life_stage | WBls:0000023 | ||||
| WBls:0000041 | |||||
| WBbt:0006749 | Certain | ||||
| Life_stage | WBls:0000017 | ||||
| Remark | Expressed in the developing nerve ring. | ||||
| WBbt:0006797 | Certain | ||||
| WBbt:0006865 | Certain | ||||
| Life_stage | WBls:0000023 | ||||
| WBls:0000041 | |||||
| GO_term | GO:0005737 | ||||
| GO:0005911 | |||||
| GO:0005938 | |||||
| Type | Antibody | ||||
| Pattern | The localization of tetraThymosin-beta is shown in oocytes (in dissected gonads of adult worms), in embryos and in whole adult organisms. TetraThymosin-beta was maternally expressed and in the adult gonad diffusely present in the cytoplasm of the oocytes, with strong staining at the cell cortex where also actin is present. In the distal arm of the gonad, tetraThymosin-beta localized to the inside edges of the membrane cubicles surrounding germ cell nuclei. Here, tetraThymosin-beta did not localize to the lateral side of the membrane cubicles where actin is arranged in a 'honeycomb' structure (Strome, 1986). In two-cell stage embryos, tetraThymosin-beta was enriched in the cell-cell contact where actin was also concentrated. The tetraThymosin-beta signal was clearly apparent until the four-cell stage and became weaker later on. At the comma stage (290 min after the first cell division), tetraThymosin-beta staining was observed at the developing nerve ring that is the largest axonal bundle in the nematode body and that positions around the isthmus of the pharynx. TetraThymosin-beta was evident in this region even before a clear actin signal was apparent. In larvae and adults, immunofluorescence yielded a diffuse but specific staining of the entire worm body with enrichment at the intestinal tract and spermatheca. At these stages, in contrast to what is observed in developing embryo, no prominent signal was observed in the head region where the nerve ring is located. In young adults is expressed in theposterior bulb of the pharynx. These immunostaining patterns were reproducible in three or more experiments and in 20 or more worms or embryos. | ||||
| Picture | WBPicture0000013342 | ||||
| WBPicture0000013343 | |||||
| Reference | WBPaper00023907 | ||||
| Antibody_info | WBAntibody00001843 |
