WormBase Tree Display for Expr_pattern: Expr1203
expand all nodes | collapse all nodes | view schema
| Expr1203 | Expression_of | Gene | WBGene00003785 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003785 | |||
| Expression_data | Life_stage | WBls:0000003 | ||
| WBls:0000023 | ||||
| Anatomy_term | WBbt:0004421 | Certain | ||
| WBbt:0004575 | Certain | |||
| WBbt:0004576 | Certain | |||
| WBbt:0005784 | Certain | |||
| WBbt:0006873 | Certain | |||
| WBbt:0006874 | Certain | |||
| WBbt:0006875 | Certain | |||
| GO_term | GO:0005737 | |||
| Subcellular_localization | At all stages of germ-line development, NOS-3 was predominantly cytoplasmic: this was evidenced by the dark, non-staining `holes' corresponding to nuclei in the germ line at the larval L3 stage. The distribution of NOS-3 overlapped with regions of the cytoplasm containing P granules, detected using anti-PGL-1 antibodies; however, within the cytoplasm, NOS-3 staining was uniform and diffuse throughout. At the late L4 stage, NOS-3 was detected in germ cells in mitosis and meiotic pachytene, but decreased in the spermatogenic region. Although the distribution of NOS-3 in P cells was punctate, it did not precisely coincide with P granules. | |||
| Type | Antibody | Affinity purified rat polyclonal antibody. | ||
| Pattern | NOS-3 protein detected throughout the germ-line tissue during larval development. In addition to staining in the larval gonad, NOS-3 expression was also observed in embryos. Specifically, NOS-3 was found in P1P4, and later in germ-line progenitor cells, Z2 and Z3. | |||
| Picture | WBPicture0000009691 | |||
| Remark | Staining with anti-NOS-3 antibodies was specific, in that it was dramatically reduced in nos-3 deletion mutants. | |||
| Reference | WBPaper00003692 | |||
| Antibody_info | WBAntibody00000240 |
