WormBase Tree Display for Expr_pattern: Expr10566
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| Expr10566 | Expression_of | Gene | WBGene00006438 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006438 | |||
| Expression_data | Life_stage | WBls:0000041 | ||
| WBls:0000023 | ||||
| Anatomy_term | WBbt:0005772 | Certain | ||
| WBbt:0005775 | Certain | |||
| GO_term | GO:0005902 | |||
| GO:0016020 | ||||
| Subcellular_localization | Using a specific antibody raised against the NRFL-1 (C01F6.6a) protein, endogenous NRFL-1 was detected in the luminal side of the intestine. However, the excretory canal was not stained with the antibody. NRFL-1 is found apical to IFB-2 (Intermediate Filament, B family-2) which localizes just beneath the intestinal microvilli, suggesting the enrichment in the intestinal microvilli. Immunofluorescence staining using anti-NRFL-1 antibody detected NRFL-1 on the intestinal luminal membrane. | |||
| Type | Antibody | |||
| Reporter_gene | ||||
| Pattern | gfp::nrfl-1 was expressed in the excretory canal, the worm counterpart of the mammalian renal tubules, and the intestine. Although previous large-scale gene expression profiling efforts using nrfl-1-promoter::gfp constructs observed GFP signals in the pharynx, intestine, excretory cells, and some tail cells, the gfp::nrfl-1 construct was exclusively expressed in the intestine and the excretory system. This pattern stayed consistent throughout the larval and adult stages. | |||
| Picture | WBPicture0000012806 | |||
| WBPicture0000012809 | ||||
| Reference | WBPaper00041455 | |||
| Transgene | WBTransgene00016059 | |||
| Antibody_info | WBAntibody00002351 |
