WormBase Tree Display for Expr_pattern: Expr10040
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| Expr10040 | Expression_of | Gene | WBGene00018350 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00018350 | |||
| Expression_data | Anatomy_term | WBbt:0005772 | Certain | |
| GO_term | GO:0016323 | |||
| GO:0016324 | ||||
| Type | Antibody | |||
| Reporter_gene | ||||
| Pattern | The IFO-1 reporters labeled the perilumenal, subapical cytoplasm of intestinal cells where they co-distributed with IFB-2. To examine the expression of endogenous IFO-1, antibodies were generated against peptides 1 (position 19-39) and 2 (position 386-405). These antibodies stained the intestine in the same fashion as the reporters and anti-IFB-2 antibodies.The establishment of polarized IFO-1 distribution was examined. At a time at which the diffuse cytoplasmic IFB-2 distribution becomes apically localized, IFO-1 fluorescence was also detectable in the same region, actually slightly preceding apical IFB-2 deposition. Apical IFO-1 increases in the subsequent 1.5-fold stage were coincident with significant IFO-1 staining of the basolateral cortex. By contrast, IFB-2 was not enriched at the basolateral cortex. At the 2- to 3-fold stage, both proteins were almost completely colocalized at the apical membrane domain. | |||
| Reference | WBPaper00040999 | |||
| Transgene | WBTransgene00015425 | |||
| Antibody_info | WBAntibody00002329 | |||
| WBAntibody00002330 |
