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WormBase Tree Display for Construct: WBCnstr00042621

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Name Class

WBCnstr00042621Public_namepRD21
Summary[Pflp-18::ctl-2::eGFP::let-858]
Driven_by_geneWBGene00001461
GeneWBGene00000831
UTR_3WBGene00002957
Fusion_reporterEGFP
Construction_summaryThe catalase gene (ctl-2) was cloned from C. elegans cDNA using the forward primer 5'-GGGGACAAGTTTGTACAAAAAAGCAGGCTATGCCAAACGATCCATCGGA-3' and reverse primer 5'-GGGGACCACTTTGTACAAGAAAGCTGGGTCGATATGAGAGCGA GCCTGTTTC-3' designed on ApE (v.2.0.60). Using the gateway recombination cloning technique (Invitrogen), the ctl-2 gene was positioned behind Pflp-18, an AVA specific promoter, and followed by an eGFP 3'UTR (from pGH112, Erik Jorgensen) in the destination vector pCFJ150 (Erik Jorgensen, Addgene - Plasmid #19329).
Used_forTransgene_constructWBTransgene00032784
ReferenceWBPaper00062470