WormBase Tree Display for Construct: WBCnstr00042621
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WBCnstr00042621 | Public_name | pRD21 | |
---|---|---|---|
Summary | [Pflp-18::ctl-2::eGFP::let-858] | ||
Driven_by_gene | WBGene00001461 | ||
Gene | WBGene00000831 | ||
UTR_3 | WBGene00002957 | ||
Fusion_reporter | EGFP | ||
Construction_summary | The catalase gene (ctl-2) was cloned from C. elegans cDNA using the forward primer 5'-GGGGACAAGTTTGTACAAAAAAGCAGGCTATGCCAAACGATCCATCGGA-3' and reverse primer 5'-GGGGACCACTTTGTACAAGAAAGCTGGGTCGATATGAGAGCGA GCCTGTTTC-3' designed on ApE (v.2.0.60). Using the gateway recombination cloning technique (Invitrogen), the ctl-2 gene was positioned behind Pflp-18, an AVA specific promoter, and followed by an eGFP 3'UTR (from pGH112, Erik Jorgensen) in the destination vector pCFJ150 (Erik Jorgensen, Addgene - Plasmid #19329). | ||
Used_for | Transgene_construct | WBTransgene00032784 | |
Reference | WBPaper00062470 |