WormBase Tree Display for Construct: WBCnstr00042251
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| WBCnstr00042251 | Summary | nhr-67p::NHR-67::TagRFP-T::AID | |
|---|---|---|---|
| Driven_by_gene | WBGene00003657 | ||
| Gene | WBGene00003657 | ||
| Fusion_reporter | tagRFP | ||
| Other_reporter | AID | ||
| Construction_summary | TagRFP-T::AID was inserted into the C-terminus of the endogenous nhr-67 locus via CRISPR/Cas9 mediated genome engineering using the self-excising cassette method (Dickinson et al., 2013; Dickinson et al., 2015). The sgRNA targeting sequence in pDD122 was replaced with the sgRNA targeting the C-terminus of nhr-67 using Gibson cloning (Dickinson etal., 2013). The repair template for nhr-67 was generated by cloning homology arms, synthesized by Twist Biosciences(left homology arm) and by PCR using genomic DNA as a template (right homology arm), into pTNM063 (TagRFPT::AID repair template) (Ashley et al., 2021). | ||
| Used_for | Transgene_construct | WBTransgene00032513 | |
| Reference | WBPaper00061826 |
