WormBase Tree Display for Construct: WBCnstr00042125
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WBCnstr00042125 | Summary | [Phsp::HBx(E126A)] |
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Driven_by_gene | WBGene00002018 | |
Other_reporter | Hepatitis B virus X protein with Glutamate 126 mutated to Alanine | |
Construction_summary | The HBx cDNA clone was kindly provided by Xiao-Kun Zhang (Burnham Institute for Medical Research, La Jolla, CA). To make PhspHBx constructs, full-length HBx cDNA was subcloned into C. elegans heat-shock vectors, pPD49.78 and pPD49.83, via NheI and KpnI sites... The HBx mutant constructs containing G124L, I127A, E125A, or E126A substitutions were generated using a QuikChange Site-Directed Mutagenesis kit (Stratagene) and confirmed by DNA sequencing. | |
Reference | WBPaper00041673 |