WormBase Tree Display for Construct: WBCnstr00040178
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WBCnstr00040178 | Summary | [Ptph-1::bas-1::gfp] | |
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Driven_by_gene | WBGene00006600 | ||
Gene | WBGene00000239 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Cterminal_translational_fusion | ||
Construction_summary | "The ... bas-1 gene (was) amplified by PCR from genomic DNA of wild-type N2 worm by the following primers: ... forward, 5'-acccccgggatggactcccaaaaattgcg-3' and reverse, 5'-gaactcgccgagaaattattcctaccggtgc-3' for bas-1 gene. To construct Ptph-1::bas-1::gfp ..., the bas-1 promoter was replaced by 3.1 kb tph-1 ... gene upstream promoters... The primers for Ptph-1 are as follows: forward, 5'-acatgcatgctcggtggtcttcccgcttgc-3' and reverse, 5'-ccccccgggatgattgaagagagcaatgc-3'" | ||
Clone | pPD95.75 | ||
Used_for | Transgene_construct | WBTransgene00025431 | |
Reference | WBPaper00044984 | ||
Laboratory | SQC |