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WormBase Tree Display for Construct: WBCnstr00022888

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Name Class

WBCnstr00022888Summary[hlh-11::GFP]
Driven_by_geneWBGene00001955
GeneWBGene00001955
Fusion_reporterGFP
Type_of_constructTranslational_fusion
Construction_summary[hlh-11::GFP] translational fusion. To obtain a full-length of hlh-11, the genomic DNA fragment containing 5,353 bp of upstream sequence and the full length of hlh-11 gene product was amplified by nested PCR using the following primer sets based on F58A4.7; outer upstream primer (5'-TCG AGA TAA CTT TGG AAA GTG CAG TG-3'), outer downstream primer (5'-GAT ACT GTG CCT GGT GAG AGA GAG-3') and inner upstream primer (5'-GGC GAT TAT TCA AGC AGG TCT AGA-3'), inner downstream primer (5'- ACG GAC CCG GGC GAG CGA TGT CTG- 3'). The amplified product was subcloned into pGEM-T easy vector (Promega, USA) and sequenced. Translational hlh-11 gene was fused to a promoterless green fluorescence protein (GFP) vector, pPD95.75 (kindly provided by A. Fire) using the XbaI and SmaI sites.
Used_forTransgene_constructWBTransgene00031961
ReferenceWBPaper00035331