WormBase Tree Display for Construct: WBCnstr00022888
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WBCnstr00022888 | Summary | [hlh-11::GFP] | |
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Driven_by_gene | WBGene00001955 | ||
Gene | WBGene00001955 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Translational_fusion | ||
Construction_summary | [hlh-11::GFP] translational fusion. To obtain a full-length of hlh-11, the genomic DNA fragment containing 5,353 bp of upstream sequence and the full length of hlh-11 gene product was amplified by nested PCR using the following primer sets based on F58A4.7; outer upstream primer (5'-TCG AGA TAA CTT TGG AAA GTG CAG TG-3'), outer downstream primer (5'-GAT ACT GTG CCT GGT GAG AGA GAG-3') and inner upstream primer (5'-GGC GAT TAT TCA AGC AGG TCT AGA-3'), inner downstream primer (5'- ACG GAC CCG GGC GAG CGA TGT CTG- 3'). The amplified product was subcloned into pGEM-T easy vector (Promega, USA) and sequenced. Translational hlh-11 gene was fused to a promoterless green fluorescence protein (GFP) vector, pPD95.75 (kindly provided by A. Fire) using the XbaI and SmaI sites. | ||
Used_for | Transgene_construct | WBTransgene00031961 | |
Reference | WBPaper00035331 |