WormBase Tree Display for Construct: WBCnstr00022883
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WBCnstr00022883 | Public_name | pCBD156 | |
---|---|---|---|
Summary | [bah-1::GFP] | ||
Driven_by_gene | WBGene00050875 | ||
Gene | WBGene00050875 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Translational_fusion | ||
Construction_summary | [bah-1::GFP] transcriptional fusion. 2 kb upstream of bah-1 was PCR amplified with primers 5'-CTGCAGGCGAACCTTAACTGGGGAGTAC and 5'-GGATCCTGTAATCAATATATGCCTTG that added restriction sites PstI and BamHI, respectively. The PCR product was ligated into vector pPD95.81 (Addgene plasmid 1497) to make pCBD156. | ||
Clone | pPD95.81 | ||
Used_for | Transgene_construct | WBTransgene00031958 | |
Reference | WBPaper00035152 |