WormBase Tree Display for Construct: WBCnstr00020798
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WBCnstr00020798 | Summary | [CYK-4::GFP::pie-1 3'UTR] | |
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Driven_by_gene | WBGene00000875 | ||
Gene | WBGene00000875 | ||
UTR_3 | WBGene00004027 | ||
Fusion_reporter | GFP | ||
Construction_summary | ~2kb sequences upstream of cyk-4, cyk-4 genomic DNA tagged with C-terminal GFP coding sequences, and pie-1 3'UTR sequences were generated by overlapping PCR and inserted to pCFJ150 by SLiCE (Zhang et al., 2012). cyk-4 genomic sequences between BamHI and AvrII were recoded to generated RNAi resistant alleles. | ||
Used_for | Transgene_construct | WBTransgene00021184 | |
Reference | WBPaper00048425 |