WormBase Tree Display for Construct: WBCnstr00020253
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| WBCnstr00020253 | Public_name | pRA568 | |
|---|---|---|---|
| Summary | [Pswsn-9::swsn-9::GFP] | ||
| Driven_by_gene | WBGene00007256 | ||
| Gene | WBGene00007256 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | The swsn-9 promoter and coding sequence, including 812 bp upstream of the ATG through the final coding amino acid, was amplified using PCR. The PCR product was cloned via engineered SphI and AgeI sites into pPD95.75 (Addgene plasmid 1494). PCR was performed with the iProof High Fidelity DNA Polymerase (Bio-Rad), and the resulting clones were sequenced to ascertain that no mutations were introduced. | ||
| Used_for | Transgene_construct | WBTransgene00020725 | |
| Reference | WBPaper00046494 |
