WormBase Tree Display for Construct: WBCnstr00020229
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| WBCnstr00020229 | Summary | [Pbcl-7::bcl-7::mCherry] | |
|---|---|---|---|
| Driven_by_gene | WBGene00016192 | ||
| Gene | WBGene00016192 | ||
| Fusion_reporter | mCherry | ||
| Construction_summary | To determine the expression pattern of the rescue plasmid in the wild-type hermaphrodite, the bcl-7 genomic sequence,comprising a 0.3-kbp sequence upstream from the ATG initiation codon of bcl-7, as well as the full-length bcl-7 (0.7 kbp), was PCR amplified from N2 genomic DNA using the following primers: bcl-7_sense, 5'-GGT TCC GCG TGG ATC CCA TTT TGA CGCAAG ATT TGA GAG-3'; bcl-7_antisense, 5'-GCT CAC CATGCG GCC GCA TGG TTG TTT TGA TGT CAT TTC A-3'. The pFX_mCherry expression vector was digested with BamHI and NotI, and the bcl-7 fragment (Pbcl-7::bcl-7) was cloned into the vector to generate Pbcl-7::bcl-7::mCherry. | ||
| Used_for | Transgene_construct | WBTransgene00020589 | |
| Reference | WBPaper00046304 |
