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WormBase Tree Display for Construct: WBCnstr00020228

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Name Class

WBCnstr00020228Summary[Pbcl-7::bcl-7::egfp]
Driven_by_geneWBGene00016192
GeneWBGene00016192
Fusion_reporterEGFP
Construction_summaryTo determine the expression pattern of the rescue plasmid in the wild-type hermaphrodite, the bcl-7 genomic sequence,comprising a 0.3-kbp sequence upstream from the ATG initiation codon of bcl-7, as well as the full-length bcl-7 (0.7 kbp), was PCR amplified from N2 genomic DNA using the following primers: bcl-7_sense, 5'-GGT TCC GCG TGG ATC CCA TTT TGA CGCAAG ATT TGA GAG-3'; bcl-7_antisense, 5'-GCT CAC CATGCG GCC GCA TGG TTG TTT TGA TGT CAT TTC A-3'. The pFX_egfp expression vector was digested with BamHI and NotI, and the bcl-7 fragment (Pbcl-7::bcl-7) was cloned into the vector to generate Pbcl-7::bcl-7::egfp.
Used_forTransgene_constructWBTransgene00020588
ReferenceWBPaper00046304