WormBase Tree Display for Construct: WBCnstr00019384
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WBCnstr00019384 | Summary | [Psft-4::GFP::SFT-4] | |
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Driven_by_gene | WBGene00004788 | ||
Gene | WBGene00004788 | ||
Fusion_reporter | GFP | ||
Construction_summary | A 2.6 genomic fragment upstream of sft-4 was amplified with primers OEH-41 and OEH-47. A 2 kb fragment containing the ORF, introns and annotated 3'UTR of sft-4 was amplified with primers OEH-44 and OEH-45. A 1 kb fragment from vector pPD95.75 containing gfp was amplified with primers OJE-05 and OEH-48. The three fragments were assembled by fusion PCR using nested primers OEH-42 and OEH-46 in the following order: 2.7 kb promoter fragment/1 kb gfp/2 kb sft-4 genomic. | ||
Used_for | Transgene_construct | WBTransgene00020059 | |
Reference | WBPaper00045219 |