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WormBase Tree Display for Construct: WBCnstr00017426

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Name Class

WBCnstr00017426Other_nameExpr10655_Ex
Summary[Pfaah-1::gfp]
Driven_by_geneWBGene00015047
Fusion_reporterGFP
Type_of_constructTranscriptional_fusion
Construction_summaryTo make Pfaah-1::gfp, a 3-kb genomic DNA of the faah-1 promoter region in N2 was amplified by PCR with the following primers:F1: 5'-GCATGCgaatcttcatcctctgatggctgcat-3'; R1: 5'-GGTACCgatgaccttgaaatactgaaaattgaaagtg-3'.The primers introduced an SphI (forward primer) and a KpnI (reverse primer) endonuclease recognition sites. Using the SphI and KpnI sites, the amplified faah-1 promoter DNA was inserted into the pPD95.75 vector. --Precise ends.
Used_forTransgene_constructWBTransgene00018036
ReferenceWBPaper00041646