WormBase Tree Display for Construct: WBCnstr00017426
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WBCnstr00017426 | Other_name | Expr10655_Ex | |
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Summary | [Pfaah-1::gfp] | ||
Driven_by_gene | WBGene00015047 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Transcriptional_fusion | ||
Construction_summary | To make Pfaah-1::gfp, a 3-kb genomic DNA of the faah-1 promoter region in N2 was amplified by PCR with the following primers:F1: 5'-GCATGCgaatcttcatcctctgatggctgcat-3'; R1: 5'-GGTACCgatgaccttgaaatactgaaaattgaaagtg-3'.The primers introduced an SphI (forward primer) and a KpnI (reverse primer) endonuclease recognition sites. Using the SphI and KpnI sites, the amplified faah-1 promoter DNA was inserted into the pPD95.75 vector. --Precise ends. | ||
Used_for | Transgene_construct | WBTransgene00018036 | |
Reference | WBPaper00041646 |